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Human ECE-2 Antibody

Catalog #: MAB16451
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MAB16451
MAB16451-SP
ECE‑2 in Human Brain.
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Human ECE-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ECE-2 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) ECE‑1 and no cross-reactivity with rhKELL is observed.
Source
Monoclonal Rat IgG2A Clone # 233914
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ECE-2
Val104-Leu787
Accession # O60344
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ECE‑2 (Catalog # 1645-ZN)
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry ECE‑2 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

ECE‑2 in Human Brain. ECE-2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 3 µg/mL Rat Anti-Human ECE-2 Monoclonal Antibody (Catalog # MAB16451) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ECE-2

ECE-2 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-1, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-2 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Three alternatively spliced forms differ in their cytoplasmic tail (2). In addition to big endothelin-1, ECE-2 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, substance P, dynorphin B, proenkephalin-derived peptides such as peptide E, BAM 22 and BAM18, and PEN-LEN, an endogenous inhibitor of prohormone convertase 1 (3). Together with ECE-1, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-2 was expressed with an N-terminal His tag and purified.

References
  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Lorenzo, M.N. et al. (2001) Biochim. Biophys. Acta 1522:46.
  3. Mzhavia, N. et al. (2003) J. Biol. Chem. 278:14704.
  4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.
Long Name
Endothelin-converting Enzyme-2
Entrez Gene IDs
9718 (Human)
Alternate Names
EC 3.4.24; EC 3.4.24.71; ECE2; ECE-2; endothelin converting enzyme 2; endothelin-converting enzyme 2; KIAA0604MGC2408; MGC17664; MGC78487

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Citation for Human ECE-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta.
    Authors: Palmer JC, Baig S, Kehoe PG, Love S
    Am. J. Pathol., 2009-06-18;175(1):262-70.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: ELISA Development, IHC-P

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