Home / EN-RAGE/S100A12 / Human EN-RAGE/S100A12 Antibody

Human EN-RAGE/S100A12 Antibody

Catalog #: AF1052
7 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1052
AF1052-SP
Detection of Human EN‑RAGE/S100A12 by Western Blot.
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Product Details
Citations (7)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EN-RAGE/S100A12 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EN-RAGE in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EN-RAGE/S100A12
Met1-Glu92
Accession # P80511
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
Human tonsil
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood monocytes
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EN-RAGE/S100A12 antibody by Western Blot. View Larger

Detection of Human EN‑RAGE/S100A12 by Western Blot. Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN-RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for EN-RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry EN‑RAGE/S100A12 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

EN‑RAGE/S100A12 in Human Tonsil. EN‑RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western View Larger

Detection of Human EN‑RAGE/S100A12 by Simple WesternTM. Simple Western lane view shows lysates of human tonsil, loaded at 0.2 mg/mL. A specific band was detected for EN‑RAGE/S100A12 at approximately 11 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Flow Cytometry View Larger

Detection of EN‑RAGE/S100A12 in Human peripheral blood monocytes by Flow Cytometry Human peripheral blood monocytes were stained with Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EN-RAGE/S100A12

EN-RAGE, also known as S100A12 and Calgranulin C, is a 10 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of EF-hand calcium-binding proteins. Like other S100 proteins, S100A12 is small and generally acidic (1-3). EN-RAGE forms noncovalent homodimers in the absence of divalent cations (4-6); calcium and zinc promote the formation of higher order assemblies including tetramers and hexamers (4, 7, 8). The ability of S100A12 to chelate zinc enables it to inhibit the zinc-dependent metalloproteases MMP-2, -3, and -9 (8). S100A12 also forms heterodimers with S100A9 and binds to RAGE (Receptor for Advanced Glycation End-products), Annexin V, and several cytosolic enzymes involved in energy metabolism (9, 10). The hexameric form of EN-RAGE in particular binds RAGE with high affinity (7). EN-RAGE induces a variety of inflammatory responses including the in vivo recruitment of neutrophils, monocytes, and mast cells and the activation of mast cells and vascular endothelial cells (9, 11-14). EN-RAGE is found at elevated levels under inflammatory conditions such as asthma, gout, rheumatoid arthritis synovial fluid, and atherosclerosis (8, 12, 14). S100A12 also promotes neurite outgrowth in isolated hippocampal neurons (15). An ortholog of S100A12 has not been identified in rodents, but the human protein is functional in mice and rats (11-16).

References
  1. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.
  2. Leclerc, E. et al. (2009) Biochim. Biophys. Acta 1793:993.
  3. Wicki, R. et al. (1996) Cell Calcium 20:459.
  4. Moroz, O.V. et al. (2009) BMC Biochem. 10:11.
  5. Miranda, L.P. et al. (2001) FEBS Lett. 488:85.
  6. Vogl, T. et al. (1999) J. Biol. Chem. 274:25291.
  7. Xie, J. et al. (2007) J. Biol. Chem. 282:4218.
  8. Goyette, J. et al. (2009) J. Immunol. 183:593.
  9. Hatakeyama, T. et al. (2004) Eur. J. Biochem. 271:3765.
  10. Hofmann, M.A. et al. (1999) Cell 97:889.
  11. Yang, Z. et al. (2001) J. Leukoc. Biol. 69:986.
  12. Rouleau, P. et al. (2003) Clin. Immunol. 107:46.
  13. Yan, W.X. et al. (2008) J. Biol. Chem. 283:13035.
  14. Yang, Z. et al. (2007) J. Allergy Clin. Immunol. 119:106.
  15. Mikkelsen, S.E. et al. (2001) J. Neurochem. 79:767.
  16. Fuellen, G. et al. (2004) OMICS 8:334.
Long Name
Extracellular Newly Identified RAGE-binding Protein
Entrez Gene IDs
6283 (Human)
Alternate Names
CAAF1; CAAF1Neutrophil S100 protein; CAAFI; CAGC; CAGCS100 calcium binding protein A12 (calgranulin C); CAGCS100; Calcium-binding protein in amniotic fluid 1; Calgranulin C; calgranulin-C; CGRPEN-RAGE; ENRAGE; EN-RAGE; Extracellular newly identified RAGE-binding protein; MRP6; p6calgranulin C; protein S100-A12; S100 calcium binding protein A12; S100 calcium-binding protein A12 (calgranulin C); S100 calcium-binding protein A12; S100A12

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Citations for Human EN-RAGE/S100A12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Application of new host biomarker profiles in quantitative point-of-care tests facilitates leprosy diagnosis in the field
    Authors: Anouk van Hooij, Susan van den Eeden, Renate Richardus, Elisa Tjon Kon Fat, Louis Wilson, Kees L.M.C. Franken et al.
    EBioMedicine
  2. AGE-RAGE interaction in the TGF?2-mediated epithelial to mesenchymal transition of human lens epithelial cells.
    Authors: Cibin T Raghavan, Ram H Nagaraj
    Glycoconjugate Journal, 2016-06-04;0(0):1573-4986.
  3. Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein).
    Authors: Shimamoto, Seiko, Kubota, Yasuo, Yamaguchi, Fuminori, Tokumitsu, Hiroshi, Kobayashi, Ryoji
    J Biol Chem, 2013-01-23;288(10):7158-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis.
    Clin. Cancer Res., 2012-06-20;18(16):4356-64.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells.
    Authors: Sharma I, Dhawan V, Mahajan N, Saha SC, Dhaliwal LK
    Fertil. Steril., 2009-11-26;94(5):1639.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Host biomarker-based quantitative rapid tests for detection and treatment monitoring of tuberculosis and COVID-19
    Authors: Pierneef L, van Hooij A, de Jong D et al.
    iScience
  7. Insights into binding of S100 proteins to scavenger receptors: class B scavenger receptor CD36 binds S100A12 with high affinity
    Authors: Christoph Tondera, Markus Laube, Jens Pietzsch
    Amino Acids

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Human EN-RAGE/S100A12 Antibody
By Anonymous on 09/29/2023
Application: WB Sample Tested: Recombinant protein Species: Human
Western Blot Human EN-RAGE/S100A12 Antibody AF1052