Human ENPP-7/Alk-SMase Antibody

Catalog #: AF4924 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4924
AF4924-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ENPP-7/Alk-SMase Antibody Summary

Species Reactivity

Human

Specificity

Detects human ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse ENPP-7 is observed, and less than 5% cross-reactivity with recombinant human (rh) ENPP-2 and rhENPP-5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP‑7/Alk‑SMase
Ala22-Ser439
Accession # Q6UWV6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ENPP‑7/Alk-SMase (Catalog # 4924-EN)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ENPP‑7/Alk-SMase (Catalog # 4924-EN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ENPP-7/Alk-SMase

ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (alk-SMase) is expressed in the intestines and in human bile (1). It shares 30%‑36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP-7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies also show a decrease in ENPP-7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP-7 in human colon cancer (4, 5).

References

Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
Wu, J. et al. (2006) Biochem J. 394:299.
Hertervig, E. et al. (1996) Cancer. 79:448.
Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 7

Entrez Gene IDs

339221 (Human); 238011 (Mouse); 303729 (Rat)

Alternate Names

Alkaline sphingomyelin phosphodiesterase; alkaline sphingomyelinase; Alk-SMase; EC 3.1.4.12; ectonucleotide pyrophosphatase/phosphodiesterase 7; ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; ENPP7; ENPP-7; Intestinal alkaline sphingomyelinase; MGC50179; NPP7; NPP-7