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Human Frizzled-6 Antibody

Catalog #: AF3149
1 Citation 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3149
AF3149-SP
Frizzled‑6 in Human Kidney Cancer Tissue.
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Frizzled-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Frizzled-6 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Frizzled-6
His19-Val153
Accession # O60353
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Frizzled‑6
Flow Cytometry
2.5 µg/106 cells
HEK293 human embryonic kidney cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Frizzled-6 antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Frizzled‑6 in Human Kidney Cancer Tissue. Frizzled-6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-6

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Entrez Gene IDs
8323 (Human); 14368 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 6; frizzled homolog 6 (Drosophila); Frizzled6; Frizzled-6; FZ6; fz-6; FZD6; Hfz6; seven transmembrane helix receptor

Product Datasheets

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Citation for Human Frizzled-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines.
    Authors: Garcia-Castro B, Alvarez-Zavala M, Riveros-Magana A, Ortiz-Lazareno P, Ratkovich-Gonzalez S, Hernandez-Flores G, Bravo-Cuellar A, Jave-Suarez L, Aguilar-Lemarroy A
    BMC Cancer, 2013-11-25;13(0):557.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Reviews for Human Frizzled-6 Antibody

Average Rating: 5 (Based on 4 Reviews)

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Human Frizzled-6 Antibody
By Ruchi Shah on 03/21/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: corneal tissue Species: Human

1% formalin fixed
0.5% Triton-X100 permeabilized
5% BSA blocking

Immunocytochemistry/Immunofluorescence Human Frizzled-6 Antibody AF3149
Human Frizzled‑6 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: MDA-MBA-231 human breast cancer cell line Species: Human

Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS with 2%BSA
Dilution: 1:250

Immunocytochemistry/Immunofluorescence Human Frizzled‑6 Antibody AF3149
Human Frizzled‑6 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: SUM-159PT human breast cancer cell line Species: Human

4% PFA fixation, Secondary Ab: Alexa488-donkey-anti-goat 1/500.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS/Tween
Dilution: 1/250

Human Frizzled‑6 Antibody
By Anonymous on 10/26/2015
Application: Immunocytochemistry/Immunofluorescence Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Cells were fixed in 4% PFA. Secondary Ab: Alexa488-donkey-anti-goat 1/500.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS/Tween
Dilution: 1/250