Home / GPR34 / Human GPR34 Antibody

Human GPR34 Antibody

Catalog #: MAB4617
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4617
MAB4617-SP
GPR34 in A172 Human Cell Line.
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GPR34 Antibody Summary

Species Reactivity
Human
Specificity
Stains human GPR34 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 419859
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR34
Accession # Q9UPC5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry GPR34 antibody in A172 Human Cell Line by Immunocytochemistry (ICC). View Larger

GPR34 in A172 Human Cell Line. GPR34 was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of GPR34 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of GPR34 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR34

GPR34 is a widely expressed 75-90 kDa seven transmembrane segment glycoprotein. It is upregulated on microglia during inflammation and functions as a receptor for lysophosphatidyl-L-serine on mast cells to promote degranulation. Multiple isoforms of GPR34 may result from multiple translation initiation sites and alternative splicing. Human GPR34 shares 89% amino acid sequence identity with mouse and rat GPR34.

Long Name
G Protein-coupled Receptor 34
Entrez Gene IDs
2857 (Human); 23890 (Mouse); 554353 (Rat)
Alternate Names
G protein-coupled receptor 34; GPR34; probable G-protein coupled receptor 34

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human GPR34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states
    Authors: NM Dräger, SM Sattler, CT Huang, OM Teter, K Leng, SH Hashemi, J Hong, G Aviles, CD Clelland, L Zhan, JC Udeochu, L Kodama, AB Singleton, MA Nalls, J Ichida, ME Ward, F Faghri, L Gan, M Kampmann
    Nature Neuroscience, 2022-08-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  2. Lysophosphatidylserine Induces MUC5AC Production via the Feedforward Regulation of the TACE-EGFR-ERK Pathway in Airway Epithelial Cells in a Receptor-Independent Manner
    Authors: MS Sim, HJ Kim, SH Jo, C Kim, IY Chung
    International Journal of Molecular Sciences, 2022-03-31;23(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Genome-wide CRISPRi/a screens in human neurons link lysosomal failure to ferroptosis
    Authors: Ruilin Tian, Anthony Abarientos, Jason Hong, Sayed Hadi Hashemi, Rui Yan, Nina Dräger et al.
    Nature Neuroscience
  4. Single amino acid substitution in LC-CDR1 induces Russell body phenotype that attenuates cellular protein synthesis through eIF2? phosphorylation and thereby downregulates IgG secretion despite operational secretory pathway traffic
    Authors: H Hasegawa, A Hsu, CE Tinberg, KE Siegler, AA Nazarian, MM Tsai
    MAbs, 2017-04-05;0(0):0.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Topogenesis and cell surface trafficking of GPR34 are facilitated by positive-inside rule that effects through a tri-basic motif in the first intracellular loop.
    Authors: Hasegawa H, Patel N, Ettehadieh E, Li P, Lim A
    Biochim Biophys Acta, 2016-04-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. t(X;14)(p11;q32) in MALT lymphoma involving GPR34 reveals a role for GPR34 in tumor cell growth.
    Authors: Ansell S, Akasaka T, McPhail E, Manske M, Braggio E, Price-Troska T, Ziesmer S, Secreto F, Fonseca R, Gupta M, Law M, Witzig T, Dyer M, Dogan A, Cerhan J, Novak A
    Blood, 2012-09-10;120(19):3949-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human GPR34 Antibody

There are currently no reviews for this product. Be the first to review Human GPR34 Antibody and earn rewards!

Have you used Human GPR34 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review