Human GPR88 Antibody

Catalog #: MAB108281 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB108281-100
MAB108281-SP

Detection of GPR88 in NS0 Mouse Cell Line.

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Human GPR88 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GPR88 in ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 1039818

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human GPR88 synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

3-25 µg/mL

Immersion fixed NS0 mouse myeloma cell line and HEK293 cell line transfected with GPR88

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of GPR88 in NS0 Mouse Cell Line. GPR88 was detected in immersion fixed NS0 mouse myeloma cell line transfected with GPR88 (positive staining) and absent in NS0 mouse myeloma cell line (wild type, negative control) using Mouse Anti-Human GPR88 Monoclonal Antibody (Catalog # MAB108281) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

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Detection of GPR88 in HEK293 human cell line. GPR88 was detected in immersion fixed HEK293 human cell line transfected with GPR88 (positive staining) and absent in HEK293 cell line (wild type, negative control) using Mouse Anti-Human GPR88 Monoclonal Antibody (Catalog # MAB108281) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR88

GPR88 is an orphan receptor that probably belongs to the G-protein coupled receptor family. Ligands for this receptor still largely remain unknown but agonists have been discovered. It is a brain-specific receptor with almost exclusive expression in the striatum, being preferentially localized in medium spiny neurons. Because of its expression, GPR88 is emerging as a drug target for CNS disorders such as schizophrenia, Parkinson's disease, anxiety, and addiction. Gpr88 KO mice data suggests that striatal GPR88 receptors influence activity in a range of procedures integrated by prefrontal, orbitofrontal and anterior cingulate cortico-striatal-thalamic loops leading to altered cognitive, motivational and reward evaluation processes.

Long Name

G Protein-coupled Receptor 88

Entrez Gene IDs

54112 (Human); 64378 (Mouse); 64443 (Rat)

Alternate Names

G protein coupled receptor 88; G protein-coupled receptor 88; GPR88; G-protein coupled receptor 88; probable G-protein coupled receptor 88; STRG; Striatum-specific G-protein coupled receptor