Human ICOS Antibody Summary
Glu21-Phe141
Accession # Q9Y6W8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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ICOS in Human Tonsil. ICOS was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human ICOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF169) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICOS
Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
- Hutloff, A. et al. (1999) Nature 397:263.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Product Datasheets
Citations for Human ICOS Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Comparison of Follicular Helper T-Cell Markers with the Expression of the Follicular Homing Marker CXCR5 in Peripheral T-Cell Lymphomas-A Reappraisal of Follicular Helper T-Cell Lymphomas
Authors: Krenács, L;Krenács, D;Borbényi, Z;Tóth, E;Nagy, A;Piukovics, K;Bagdi, E;
International journal of molecular sciences
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Influence on Soluble and Membrane Bound ICOS in Combination with Immune Checkpoint Blockade
Authors: Li, X;Li, J;Zheng, Y;Lee, SJ;Zhou, J;Giobbie-Hurder, A;Butterfield, LH;Dranoff, G;Hodi, FS;
Cancer immunology research
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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