Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

Name: Human IFN-alpha 2 Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HSIFNA2
Price: 675 USD

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human IFN-alpha 2 Quantikine HS ELISA Kit

Catalog #: HSIFNA2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
HSIFNA2

Control Products Available

QC297: Quantikine Immunoassay Control 1117 for Human IFN-a 2 HS

Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity

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All IFN-alpha 2/IFNA2 ELISAs

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IFN-alpha 2 Quantikine HS ELISA Kit Summary

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

0.107 pg/mL

Assay Range

0.8 - 50 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IFN-alpha 2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine™ HS Human IFN-alpha 2 Immunoassay is a 4.0 hour solid phase ELISA designed to measure human IFN-alpha 2 (IFN-alpha 2a, IFN-alpha 2b, IFN-alpha 2c) in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-alpha 2a and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-alpha 2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-alpha 2.

Recovery

The recovery of human IFN-alpha 2 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	90-108
EDTA Plasma (n=4)	89	75-101
Heparin Plasma (n=4)	86	75-92
Serum (n=4)	86	75-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IFN-alpha 2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

ELISA

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Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity IFN-Alpha 2 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-112% for Heparin Plasma, 97%-114% for EDTA Plasma, and 94%-111% for Serum.

N/A

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Human IFN-alpha 2 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha 2/IFNA2

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name:

Interferon alpha 2

Entrez Gene IDs:

3440 (Human); 15965 (Mouse); 298213 (Rat)

Alternate Names:

alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765

Related Research Areas

Interferons

FAQs

Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
- A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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