Human IFN-gamma Antibody Summary
Met1-Gln144
Accession # Q14609
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN-gamma (285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA). The ND50 is typically 0.0300 ‑ 0.300 µg/mL.
IFN‑ gamma in Human PBMCs. IFN-gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red); (NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma
Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
Product Datasheets
Citations for Human IFN-gamma Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
19
Citations: Showing 1 - 10
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PD‑L1/PD‑1 axis serves an important role in natural killer cell‑induced cytotoxicity in osteosarcoma
Authors: Ming‑Lei Zhang, Li Chen, Yan‑Jiao Li, Da‑Liang Kong
Oncology Reports
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IL‐2 modulates Th2 cell responses to glucocorticosteroid: A cause of persistent type 2 inflammation?
Authors: Tharsan Kanagalingam, Lauren Solomon, Meerah Vijeyakumaran, Nami Shrestha Palikhe, Harissios Vliagoftis, Lisa Cameron
Immunity, Inflammation and Disease
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PD-1 marks dysfunctional regulatory T cells in malignant gliomas
Authors: Daniel E. Lowther, Brittany A. Goods, Liliana E. Lucca, Benjamin A. Lerner, Khadir Raddassi, David van Dijk et al.
JCI Insight
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Functional Analysis of an Inducible Promoter Driven by Activation Signals from a Chimeric Antigen Receptor
Authors: Ryosuke Uchibori, Takeshi Teruya, Hiroyuki Ido, Ken Ohmine, Yoshihide Sehara, Masashi Urabe et al.
Molecular Therapy - Oncolytics
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Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques
Authors: H Zheng, Y Chen, J Li, H Li, X Zhao, J Li, F Yang, Y Li, C Liu, L Qin, Y Zuo, Q Zhang, Z He, H Shi, Q Li, L Liu
Cell Reports, 2022-05-08;39(8):110864.
Species: Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Tissue
Applications: IHC -
Killer cell proteases can target viral immediate-early proteins to control human cytomegalovirus infection in a noncytotoxic manner
Authors: L Shan, S Li, J Meeldijk, B Blijenberg, A Hendriks, KJWM van Boxtel, SPH van den Be, IJ Groves, M Potts, A Svrlanska, T Stamminger, MR Wills, N Bovenschen
PLoS Pathog., 2020-04-13;16(4):e1008426.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56
Authors: F Zou, L Lu, J Liu, B Xia, W Zhang, Q Hu, W Liu, Y Zhang, Y Lin, S Jing, M Huang, B Huang, B Liu, H Zhang
Nat Commun, 2019-09-11;10(1):4109.
Species: Xenograft
Sample Types: Whole Tissue
Applications: CAR-T (IHC-Fr) -
The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice.
Authors: Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W
PLoS ONE, 2015-08-18;10(8):e0135999.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
Authors: Wang R, Jaw JJ, Jaw J, Stutzman NC, Stutzman N, Zou Z, Sun PD
J Leukoc Biol, 2011-11-01;91(2):299-309.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
PLoS Pathog., 2011-04-14;7(4):e1002016.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Immunoproteasome expression is induced in mesial temporal lobe epilepsy.
Authors: Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, Marucci G, Franceschi C
Biochem. Biophys. Res. Commun., 2011-03-30;408(1):65-70.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
Blood, 2010-06-10;116(12):2061-9.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
Immunol. Lett., 2009-11-04;127(2):150-6.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
Mucosal Immunol, 2008-07-02;1(5):412-20.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Upregulated IL-23 and IL-17 in Behcet patients with active uveitis.
Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
Invest. Ophthalmol. Vis. Sci., 2008-07-01;49(7):3058-64.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
Eur. J. Immunol., 2008-04-01;38(4):1012-23.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M
Arthritis Rheum., 2003-02-01;48(2):334-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Interleukin-25 initiates Th2 differentiation of human CD4(+) T cells and influences expression of its own receptor
Authors: Graeme Bredo, Jessica Storie, Nami Shrestha Palikhe, Courtney Davidson, Alexis Adams, Harissios Vliagoftis et al.
Immunity, Inflammation and Disease
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Sodium chloride inhibits the suppressive function of FOXP3+ regulatory T cells
Authors: Amanda L. Hernandez, Alexandra Kitz, Chuan Wu, Daniel E. Lowther, Donald M. Rodriguez, Nalini Vudattu et al.
Journal of Clinical Investigation
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Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287
Used at 10ug/ml.
Briefly, frozen brain sections were fixed in 4% PFA (Fisher Scientific), followed by antigen retrieval using heating in acid citric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector). Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc Receptor Blocking Solution was added (Human TruStain FcX Biolegend,
London, UK). Primary antibody was added overnight at 4 °C and detected with donkey anti-goat-biotin (ab6578, Abcam), followed by streptavidin-alkaline phosphatase (SA-5100, Vector) and visualised with the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector).