Human IFN-gamma R1/CD119 PE-conjugated Antibody

Catalog #: FAB673P
5 Citations Datasheet / COA / SDS

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FAB673P

Detection of IFN‑ gamma R1/CD119 in Human Blood Monocytes by Flow Cytometry.

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Citations (5)

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Human IFN-gamma R1/CD119 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta.

Source

Monoclonal Mouse IgG₁ Clone # 92101

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R1
Glu18-Gly245
Accession # P15260.1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IFN‑ gamma R1/CD119 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human IFN-gamma R1/CD119 PE-conjugated Monoclonal Antibody (Catalog # FAB673P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma receptor complex is made up of two type I transmembrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 489 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.

Long Name

Interferon gamma Receptor 1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Alternate Names

AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human IFN-gamma R1/CD119 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Regulation of PD-L1 expression in non–small cell lung cancer by interleukin-1 beta
Authors: Aiko Hirayama, Kentaro Tanaka, Hirono Tsutsumi, Takayuki Nakanishi, Sho Yamashita, Shun Mizusaki et al.
Frontiers in Immunology
An ex vivo tumor fragment platform to dissect response to PD-1 blockade in cancer
Authors: P Voabil, M de Bruijn, LM Roelofsen, SH Hendriks, S Brokamp, M van den Br, A Broeks, J Sanders, P Herzig, A Zippelius, CU Blank, KJ Hartemink, K Monkhorst, JBAG Haanen, TN Schumacher, DS Thommen
Nature Medicine, 2021-07-08;27(7):1250-1261.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Function of multiple sclerosis-protective HLA class I alleles revealed by genome-wide protein-quantitative trait loci mapping of interferon signalling
Authors: C Lundtoft, P Pucholt, J Imgenberg-, J Carlsson-A, ML Eloranta, AC Syvänen, G Nordmark, JK Sandling, I Kockum, T Olsson, L Rönnblom, N Hagberg
PLoS Genet, 2020-10-26;16(10):e1009199.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Circulating microparticle subpopulations in systemic lupus erythematosus are affected by disease activity
Authors: P López, J Rodríguez-, A Martínez-Z, L Caminal-Mo, A Suárez
Int. J. Cardiol, 2017-03-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells.
Authors: Ghirelli C, Zollinger R, Soumelis V
Blood, 2010-04-09;115(24):5037-40.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry