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Human IgA Antibody

Catalog #: MAB4787
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB4787-100
MAB4787-SP
Detection of Human IgA by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IgA Antibody Summary

Species Reactivity
Human
Specificity
Detects human IgA in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2217A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Purified IgA from human serum
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IgA antibody by Western Blot. View Larger

Detection of Human IgA by Western Blot. Western blot shows human serum, primate serum, mouse serum, and rat serum. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human IgA Monoclonal Antibody (Catalog # MAB4787) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgA at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgA

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin A
Alternate Names
IgA; Immunoglobulin A

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Citation for Human IgA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Increased Cathepsin S activity associated with decreased protease inhibitory capacity contributes to altered tear proteins in Sj�gren's Syndrome patients
    Authors: MC Edman, SR Janga, Z Meng, M Bechtold, AF Chen, C Kim, L Naman, A Sarma, N Teekappana, AY Kim, S Madrigal, S Singh, E Ortiz, S Christiana, DG Arkfeld, WJ Mack, M Heur, W Stohl, SF Hamm-Alvar
    Sci Rep, 2018-07-23;8(1):11044.
    Species: Human
    Sample Types: Tears
    Applications: Western Blot

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