Home / IgG / Human IgG Fc Antibody

Human IgG Fc Antibody

Catalog #: MAB110
11 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB110-SP
MAB110-100
MAB110-500
Detection of IgG1 Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.
1 Image
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IgG Fc Antibody Summary

Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IgG Fc region under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IgG1 Fc antibody in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of IgG1 Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. (A) Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). (B) In the absence of rhB7-H1/PD-L1 Fc Chimera, HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human IgG Fc Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Systemic delivery of a targeted synthetic immunostimulant transforms the immune landscape for effective tumor regression
    Authors: Caitlyn L. Miller, Idit Sagiv-Barfi, Patrick Neuhöfer, Debra K. Czerwinski, Steven E. Artandi, Carolyn R. Bertozzi et al.
    Cell Chemical Biology
  2. Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface
    Authors: Kummarapurugu, AB;Hawkridge, AM;Ma, J;Osei, S;Martin, RK;Zheng, S;Voynow, JA;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  3. Tumour-associated neutrophils orchestrate intratumoural IL-8-driven immune evasion through Jagged2 activation in ovarian cancer
    Authors: M Yang, G Zhang, Y Wang, M He, Q Xu, J Lu, H Liu, C Xu
    Br. J. Cancer, 2020-08-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  4. PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival
    Authors: Y Yoon, G Voloudakis, N Doran, E Zhang, C Dimovasili, L Chen, Z Shao, S Darmanis, C Tang, J Tang, VX Wang, PR Hof, NK Robakis, A Georgakopo
    Mol. Psychiatry, 2020-06-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-?
    Aging Cell, 2016-09-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses
    Authors: Wan-Chien Cheng
    Eur J Immunol, 2016-08-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  7. Role of Lactobacillus pentosus Strain b240 and the Toll-like receptor 2 axis in Peyer's patch dendritic cell-mediated immunoglobulin A enhancement.
    Authors: Kotani Y, Kunisawa J, Suzuki Y, Sato I, Saito T, Toba M, Kohda N, Kiyono H
    PLoS ONE, 2014-03-14;9(3):e91857.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization Control
  8. Epithelial junction opener JO-1 improves monoclonal antibody therapy of cancer.
    Cancer Res., 2011-10-11;71(22):7080-90.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  9. Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library
    Authors: Ivette Mendoza-Salazar, Keyla M. Gómez-Castellano, Edith González-González, Ramsés Gamboa-Suasnavart, Stefany D. Rodríguez-Luna, Giovanni Santiago-Casas et al.
    Antibodies (Basel)
  10. Amyloid-beta modulates microglial responses by binding to the triggering receptor expressed on myeloid cells 2 (TREM2)
    Authors: Li Zhong, Zongqi Wang, Daxin Wang, Zhe Wang, Yuka A. Martens, Linbei Wu et al.
    Molecular Neurodegeneration

FAQs

  1. What is the light chain of the Human IgG Fc Antibody, Catalog #s MAB110 and FAB110, Clone # 97924?

    • Catalog #s MAB110 and FAB110 have a kappa light chain.

View all Antibody FAQs
Loading...

Reviews for Human IgG Fc Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human IgG Fc Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human IgG Fc Antibody
By Anonymous on 07/01/2022
Application: WB Sample Tested: Recombinant protein Species: Human
Western Blot Human IgG Fc Antibody MAB110
Human IgG Fc Antibody
By Anonymous on 10/09/2021
Application: WB Sample Tested: HEK293 Human Cell Line Species: Human
Western Blot Human IgG Fc Antibody MAB110