Home / IL-12/IL-23 p40 / Human IL-12/IL-23 p40 MAb (Clone 169516)

Human IL-12/IL-23 p40 MAb (Clone 169516)

Catalog #: MAB6091
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6091-SP
MAB6091-500
MAB6091-100
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-12/IL-23 p40 MAb (Clone 169516) Summary

Species Reactivity
Human
Specificity
Detects human IL-12/IL-23 p40 subunit in direct ELISAs and Western blots. Recognizes IL-12 and IL-23 heterodimers as well as p40 homodimers. Shows 100% cross-reactivity with porcine IL-12/IL-23 p40 and no cross-reactivity with mouse IL-12/IL-23 p40.
Source
Monoclonal Mouse IgG2B Clone # 169516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑23 p19/p40 heterodimer
Ile23-Ser328
Accession # P29460
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑12/IL‑23 p40 Monomer (Catalog # 309-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDA (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Long Name
Interleukin 12/Interleukin 23 p40
Entrez Gene IDs
3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)
Alternate Names
CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

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Citations for Human IL-12/IL-23 p40 MAb (Clone 169516)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Cross-laboratory evaluation of multiplex bead assays including independent common reference standards for immunological monitoring of observational and interventional human studies
    Authors: KE van Meijga, B Khatri, SG Smith, AMFH Drittij, RA de Paus, JJ Goeman, MM Ho, HM Dockrell, H McShane, SA Joosten, THM Ottenhoff
    PLoS ONE, 2018-09-04;13(9):e0201205.
  2. Interleukin-12B is upregulated by decoy receptor 3 in rheumatoid synovial fibroblasts
    Authors: KOJI FUKUDA, YASUSHI MIURA, TOSHIHISA MAEDA, SHINYA HAYASHI, MASAHIRO KUROSAKA
    Molecular Medicine Reports
  3. Integration of phage and yeast display platforms: A reliable and cost effective approach for binning of peptides as displayed on-phage
    Authors: P Pandya, RO Sayers, JP Ting, S Morshedian, C Torres, JS Cudal, K Zhang, JR Fitchett, Q Zhang, FF Zhang, J Wang, JD Durbin, JJ Carrillo, A Espada, H Broughton, Y Qian, S Afshar
    PLoS ONE, 2020-06-01;15(6):e0233961.
    Species: Yeast
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
    Authors: Yu RY, Gallagher G
    J. Immunol., 2010-11-12;185(12):7302-8.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production.
    Authors: Vaknin-Dembinsky A, Balashov K, Weiner HL
    J. Immunol., 2006-06-15;176(12):7768-74.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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