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Human IL-34 Biotinylated Antibody

Catalog #: BAM52652 Datasheet / COA / SDS
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BAM52652
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-34 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-34 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse IL-34 is observed.
Source
Monoclonal Mouse IgG1 Clone # 578401
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-34
Asn21-Pro242
Accession # NP_689669
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human IL-34 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human IL‑34 Antibody (Catalog # MAB52651)

Standard: Recombinant Human IL-34 Protein (Catalog # 5265-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. The mature chain contains one potential site for N-linked glycosylation. IL-34 is widely expressed, but it is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). Functionally, IL-34 stimulates monocyte viability.

Long Name
Interleukin 34
Entrez Gene IDs
146433 (Human); 76527 (Mouse)
Alternate Names
C16orf77; IL34; IL-34; interleukin 34

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