Human IL-37/IL-1F7 Antibody Summary
Lys27-Asp192
Accession # NP_775297
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IL-37/IL-1F7 by Western Blot IL-37d inhibited pro-inflammatory cytokines expression.a–c A549 cells were transfected with the mock, IL-37b, or IL-37d recombinant plasmid, respectively, and stimulated with or without IL-1 beta (10 ng/ml) for the indicated times. The expressions of IL-37b and IL-37d were detected by RT-PCR (a) and western blot (b). The levels of IL-6 in the cell culture supernatants were determined by ELISA (c). The expression of IL-37d in bone marrow-derived macrophages (BMDMs) from wild-type and IL-37dtg mouse was detected by RT-PCR (d). BMDMs were stimulated with or without LPS (100 ng/ml) for 3 h, 6 h, or 9 h. The mRNA levels of IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha were determined by qRT-PCR (e–h) and the levels of IL-6 and TNF in the cell culture supernatants were determined by cytometric beads array (CBA) (i, j). ND, Not detectable. *P < 0.05; **P < 0.01; ***P < 0.001, ns, no significant difference. Data are shown as the mean ± SEM from three independent experiments Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41419-018-0664-0), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-37/IL-1F7
Human interleukin 1 family member 7 (IL-1F7), also named FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8).
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar S. et al. (2000) J. Biol. Chem. 275:10308.
- Busfield S.J. et al. (2000) Genomics 66:213.
- Kumar S. et al. (2002) Cytokine 18:61.
- Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
- Gao, W. et al. (2003) J. Immunol. 170:1077.
- Pan, G. et al. (2001) Cytokine 13:1.
- Sims, J.E. et al. (2001) Trends Immunol. 22:533.
Product Datasheets
Citations for Human IL-37/IL-1F7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Interleukin-37 reduces inflammation and impairs phagocytosis of platelets in immune thrombocytopenia (ITP)
Authors: Y Zhao, X Ni, P Xu, Q Liu, T Sun, X Liu, X Ji, J Qiu, J Li, S Wang, P Han, J Peng, M Hou, G Li
Cytokine, 2019-09-23;125(0):154853.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-37 isoform D downregulates pro-inflammatory cytokines expression in a Smad3-dependent manner
Authors: M Zhao, Y Li, C Guo, L Wang, H Chu, F Zhu, Y Li, X Wang, Q Wang, W Zhao, Y Shi, W Chen, L Zhang
Cell Death Dis, 2018-05-22;9(6):582.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
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