Human IRAK3 Antibody Summary
aa 1-596
Accession # Q9Y616
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IRAK3 by Western Blot. Western blot shows lysates of EOL-1 human acute myeloid (eosinophilic) leukemia cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for IRAK3 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human IRAK3 by Western Blot. Western blot shows recombinant human IRAK1, recombinant human IRAK2, recombinant human IRAK3, and recombinant human IRAK4 (2 ng/lane). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1
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Detection of Human IRAK3 by Simple WesternTM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for IRAK3 at approximately 65 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRAK3
IRAK3 (Interleukin-1 receptor-associated kinase 3; also IRAK-M) is a 68‑70 kDa member of the Pelle subfamily, TKL Ser/Thr protein kinase family of proteins. IRAK3 has limited expression, being mainly found in macrophages, eosinophils and respiratory epithelium, including type II alveolar cells. IRAK3 is a negative regulator of TLR signaling. Following TLR activation, it appears to form a complex with MyD88, IRAK1 and IRAK4 on the TLR cytoplasmic domain. This effectively blocks IRAK1/4 phosphorylation and inhibits NF kappa B and MAPK downstream activation. Human IRAK3 is 596 amino acids (aa) in length, contains one death domain (aa 41‑106) and a nonfunctional protein kinase domain (aa 171‑443). There are six Ser phosphorylation sites. There is one splice variant with a deletion of aa 45‑105.
Product Datasheets
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