Human ITIH2 Antibody

Catalog #: MAB101991 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB101991-100
MAB101991-SP

Detection of Human ITIH2 by Western Blot.

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All ITIH2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ITIH2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ITIH2 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1023658

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Spodoptera frugiperda Sf 9 (baculovirus)-derived human ITIH2
Ser55-Asp702
Accession # P19823

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HepG2 human hepatocellular carcinoma cell line

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101993).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ITIH2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101991) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ITIH2 at approximately 106 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

ELISA

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Human ITIH2 ELISA Standard Curve. Recombinant Human ITIH2 protein was serially diluted 2-fold and captured by Mouse Anti-Human ITIH2 Monoclonal Antibody (MAB101993) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101991) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITIH2

The ITIs are a family of plasma protease inhibitors contributing to extracellular matrix stabilization by covalent linkage to hyaluronan. ITIH2 is a binding protein between hyaluronan and other matrix proteins. It regulates the localization, synthesis and degradation of hyaluronan. ITIH molecules are shown to play a role in carcinogenesis and inflammation. ITIH genes are downregulated in multiple human solid tumors. This downregulation contributes to initiation and/or progression of malignancy.

Long Name

Inter-Alpha-Trypsin Inhibitor Heavy Chain 2

Entrez Gene IDs

3698 (Human); 16425 (Mouse)

Alternate Names

H2P; IGHEP2; Inter-Alpha (Globulin) Inhibitor H2; inter-alpha (globulin) inhibitor, H2 polypeptide; Inter-alpha-inhibitor heavy chain 2; Inter-alpha-trypsin inhibitor complex component II; inter-alpha-trypsin inhibitor heavy chain H2; Intin2; ITI Heavy Chain H2; ITIH2; ITI-H2; ITI-HC2; Serum-derived hyaluronan-associated protein; SHAP