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Human LMO2 Antibody

Catalog #: AF2726
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2726
AF2726-SP
Detection of LMO2 in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (15)
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Human LMO2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LMO2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LMO2
Ser2-Ile158
Accession # P25791
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LMO2
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LMO2 antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of LMO2 in K562 Human Cell Line by Flow Cytometry. K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of LMO2 antibody in Human PBMC antibody by Flow Cytometry View Larger

Detection of LMO2 in Human PBMC by Flow Cytometry Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry LMO2 antibody in K562 Human Cell Line by Immunocytochemistry (ICC). View Larger

LMO2 in K562 Human Cell Line. LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot Detection of Mouse LMO2 by Western Blot View Larger

Detection of Mouse LMO2 by Western Blot Gene expression analysis of Tal1−/− Flk-1+ cells. (A) Lmo2, Tal1 gene expression relative to Hprt in Flk-1+ cells derived from WT, Lmo2−/− or Tal1−/− ES cells. Data represent the mean of three independent samples measured in duplicate ± standard deviation. (B) LMO2, TAL1, LDB1 and beta -actin protein levels detected by western blotting using nuclear extract from WT, Lmo2−/− and Tal1−/− Flk-1+ cells. (C) Pairwise comparison of significantly differentially expressed genes. Quantitation and analyses of RNAseq data are based on biological triplicate samples. (D) Heat map showing hierarchical clustering of differentially expressed genes at the Flk-1+ stage. (E) Gene ontology enrichment analysis for biological process was performed on cluster 5 as identified in D. Terms were ordered according to their Modified Fisher Extract P-value and only terms with P < 0.05 were considered significant. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article/45/17/9874/3902958), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMO2

LMO2, also known as Rhombotin-2 and T-cell translocation protein-2, is a transcriptional co‑factor that is required for hematopoietic and endothelial development. It contains two LIM domains that are characterized by a zinc binding, cysteine rich motif consisting of two tandemly repeated zinc fingers. LMO2 does not interact directly with DNA but is involved in the assembly of multiprotein transcription factor complexes. Human and mouse LMO2 share 99% amino acid sequence homology.

Long Name
LIM Domain Only 2
Entrez Gene IDs
4005 (Human)
Alternate Names
Cysteine-rich protein TTG-2; LIM domain only 2 (rhombotin-like 1); LIM domain only protein 2; LMO2; LMO-2; RBTN2; RBTN2rhombotin-2; RBTNL1; RBTNL1rhombotin-like 1; RHOM2; RHOM2T-cell translocation protein 2; T-cell translocation gene 2; TTG2; TTG2rhombotin 2

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Citations for Human LMO2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Inhibition of G9a methyltransferase stimulates fetal hemoglobin production by facilitating LCR/gamma-globin looping.
    Authors: Krivega I, Byrnes C, de Vasconcellos J, Lee Y, Kaushal M, Dean A, Miller J
    Blood, 2015-05-15;126(5):665-72.
  2. SWI/SNF Blockade Disrupts PU.1-Directed Enhancer Programs in Normal Hematopoietic Cells and Acute Myeloid Leukemia
    Authors: Courtney Chambers, Katerina Cermakova, Yuen San Chan, Kristen Kurtz, Katharina Wohlan, Andrew Henry Lewis et al.
    Cancer Research
  3. LMO2 is essential to maintain the ability of progenitors to differentiate into T-cell lineage in mice
    Authors: KI Hirano, H Hosokawa, M Koizumi, Y Endo, T Yahata, K Ando, K Hozumi
    Elife, 2021-08-12;10(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  4. A cell-based screening method using an intracellular antibody for discovering small molecules targeting the translocation protein LMO2
    Authors: N Bery, CJR Bataille, A Russell, A Hayes, F Raynaud, S Milhas, S Anand, H Tulmin, A Miller, TH Rabbitts
    Science Advances, 2021-04-09;7(15):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Ldb1 is required for Lmo2 oncogene–induced thymocyte self-renewal and T-cell acute lymphoblastic leukemia
    Authors: LiQi Li, Apratim Mitra, Kairong Cui, Bin Zhao, Seeyoung Choi, Jan Y. Lee et al.
    Blood
  6. Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells
    Authors: M Nafria, P Keane, ES Ng, EG Stanley, AG Elefanty, C Bonifer
    Cell Rep, 2020-05-26;31(8):107691.
    Species: Human
    Sample Types:
    Applications: ChIP-Seq
  7. LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage
    Authors: VS Stanulovic, P Cauchy, SA Assi, M Hoogenkamp
    Nucleic Acids Res., 2017-09-29;45(17):9874-9888.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
    Nat Commun, 2016-11-21;7(0):13396.
    Species: Human
    Sample Types: Complex Sample Type
  9. Original Research: Generation of non-deletional hereditary persistence of fetal hemoglobin beta -globin locus yeast artificial chromosome transgenic mouse models: -175 Black HPFH and -195 Brazilian HPFH
    Authors: Carolina A Braghini, Flavia C Costa, Halyna Fedosyuk, Renee Y Neades, Lesya V Novikova, Matthew P Parker et al.
    Experimental Biology and Medicine
  10. Editing the genome to introduce a beneficial naturally occurring mutation associated with increased fetal globin
    Authors: Beeke Wienert, Alister P. W. Funnell, Laura J. Norton, Richard C. M. Pearson, Lorna E. Wilkinson-White, Krystal Lester et al.
    Nature Communications
  11. Identification of a Dynamic Core Transcriptional Network in t(8;21) AML that Regulates Differentiation Block and Self-Renewal
    Authors: Anetta Ptasinska, Salam A. Assi, Natalia Martinez-Soria, Maria Rosaria Imperato, Jason Piper, Pierre Cauchy et al.
    Cell Reports
  12. The hematopoietic regulator TAL1 is required for chromatin looping between the beta -globin LCR and human gamma -globin genes to activate transcription
    Authors: Won Ju Yun, Yea Woon Kim, Yujin Kang, Jungbae Lee, Ann Dean, AeRi Kim
    Nucleic Acids Research
  13. Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential.
    Authors: Treanor L, Zhou S, Janke L, Churchman M, Ma Z, Lu T, Chen S, Mullighan C, Sorrentino B
    J Exp Med, 2014-03-31;211(4):701-13.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Aberrant induction of LMO2 by the E2A-HLF chimeric transcription factor and its implication in leukemogenesis of B-precursor ALL with t(17;19).
    Authors: Hirose K, Inukai T, Kikuchi J, Furukawa Y, Ikawa T, Kawamoto H, Oram SH, Gottgens B, Kiyokawa N, Miyagawa Y, Okita H, Akahane K, Zhang X, Kuroda I, Honna H, Kagami K, Goi K, Kurosawa H, Look AT, Matsui H, Inaba T, Sugita K
    Blood, 2010-06-02;116(6):962-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. GFI1B controls its own expression binding to multiple sites
    Authors: Eduardo Anguita, Ana Villegas, Francisco Iborra, Aurora Hernandez
    Haematologica

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