Human LYVE-1 Antibody

Catalog #: MAB11463 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11463-100
MAB11463-SP

Detection of LYVE‑1 in NSO cell line transfected with hLYVE-1 vs irrelevant NS0 tansfectant by Flow Cytometry

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Human LYVE-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LYVE-1 in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 1072614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LYVE-1
Ser24-Thr238
Accession # Q9Y5Y7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

NS0 cell line transfected with hLYVE-1 or irrelevant NS0 transfectant

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LYVE‑1 in NSO cell line transfected with hLYVE-1 vs irrelevant NS0 tansfectant by Flow Cytometry NS0 cell line transfected with hLYVE-1 (filled histogram) or irrelevant NS0 transfectant (open histogram) were stained with Rat Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB11463) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a 60-70 kDa type I transmembrane protein that is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1‑3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9‑11).

References

Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.
Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
Banerji, S. et al. (1999) J. Cell Biol. 144:789.
Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

Long Name

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Entrez Gene IDs

10894 (Human); 114332 (Mouse); 293186 (Rat)

Alternate Names

cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; UNQ230/PRO263; XLKD1