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Human MICA Antibody

Catalog #: AF1300
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1300
AF1300-SP
MICA in Human Breast Cancer Tissue.
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Product Details
Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MICA Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICA
Ala23-Gln308
Accession # NP_001170990
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.4-1.2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of biotinylated Recombinant Human MICA Fc Chimera to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299‑NK) coated at 2 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry MICA antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MICA in Human Breast Cancer Tissue. MICA was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human MICA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1300) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICA

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+  alpha beta  T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

References
  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. NKG2D and its Ligands (2002) http://www.RnDSystems.com
Long Name
MHC Class I-related Protein A
Entrez Gene IDs
100507436 (Human)
Alternate Names
FLJ60820; MGC111087; MICA; PERB11.1

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Citations for Human MICA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Role of NKG2D, DNAM-1 and natural cytotoxicity receptors in cytotoxicity toward rhabdomyosarcoma cell lines mediated by resting and IL-15-activated human natural killer cells.
    Authors: Boerman GH, van Ostaijen-ten Dam MM, Kraal KC et al.
    Cancer Immunol Immunother
  2. Plasma biomarkers for prediction of early tumor recurrence after resection of pancreatic ductal adenocarcinoma
    Authors: MC Rittmann, S Hussung, LM Braun, RFU Klar, EA Biesel, S Fichtner-F, R Fritsch, UA Wittel, DA Ruess
    Scientific Reports, 2021-04-05;11(1):7499.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  4. Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands.
    Authors: Herrmann, Julia, Berberich, Hannah, Hartmann, Jessica, Beyer, Steffen, Davies, Karen, Koch, Joachim
    J Biol Chem, 2013-11-25;289(2):765-77.
    Species: Moth - Trichoplusia ni (Cabbage Looper)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  5. Compartmental localization and clinical relevance of MICA antibodies after renal transplantation.
    Authors: Li L, Chen A, Chaudhuri A, Kambham N, Sigdel T, Chen R, Sarwal MM
    Transplantation, 2010-02-15;89(3):312-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.
    Authors: Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
    Int. J. Cancer, 2009-04-01;124(7):1594-604.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008-08-01;181(3):2227-37.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
    Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
    Mol. Immunol., 2008-07-26;45(15):3917-25.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
    Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
    Cancer Res., 2007-08-01;67(15):7458-66.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Endothelial Cell Activation and Proliferation Modulate NKG2D Activity by Regulating MICA Expression and Shedding
    Authors: Annabelle Chauveau, Pierre Tonnerre, Angélique Pabois, Pierre-Jean Gavlovsky, Mathais Chatelais, Stéphanie Coupel et al.
    Journal of Innate Immunity

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