Human MMP-16/MT3-MMP Antibody

Catalog #: AF1785 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1785
AF1785-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-16/MT3-MMP Antibody Summary

Species Reactivity

Human

Specificity

Detects human MMP‑16/MT3‑MMP in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MMP‑16/MT3‑MMP
Ala32-Gly291 (Ile152Asn)
Accession # P51512

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MMP‑16/MT3-MMP (Catalog # 1785-MP)

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human MMP‑16/MT3-MMP (Catalog # 1785-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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MMP‑16/MT3‑MMP in Human Breast. MMP-16/MT3-MMP was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human MMP-16/MT3-MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1785) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-16/MT3-MMP

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix (ECM). MMP-16 (MT3-MMP) is found in brain, lung, placenta, smooth muscle cells, and malignant tumor tissues including oral melanoma and renal carcinoma (1). MMP-16 has been shown to activate proMMP-2 and degrade various ECM components including native collagens (2, 3). MMP-16 has been proposed to possess the potential to directly enhance the growth and invasiveness of cells in vivo, two critical processes for development and carcinogenesis (4). Structurally, MMP-16 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (1). The structure of the catalytic domain in complex with a hydroxamate inhibitor has been solved (5). The rhMMP-16PC consists of the pro and catalytic domains, which can be activated by treatment with furin.

References

Takino, T. et al. (1995) J. Biol. Chem. 270:23013.
Shofuda, K. et al. (1997) J. Biol. Chem. 272:9749.
Shimada, T. et al. (1999) Eur. J. Biochem. 262:907.
Kang, T. et al. (2000) FASEB J. 14:2559.
Lang, R. et al. (2004) J. Mol. Biol. 336:213.

Long Name

Matrix Metalloproteinase 16/Membrane Type 3 MMP

Entrez Gene IDs

4325 (Human)

Alternate Names

chromosome 8 open reading frame 57; DKFZp761D112; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 16 (membrane-inserted); matrix metalloproteinase 16 (membrane-inserted); matrix metalloproteinase-16; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP16; MMP-16; MMPX2; MMP-X2; MT3MMP; MT3-MMP; MT3-MMPC8orf57; MT-MMP 3; MT-MMP2; MTMMP3; MT-MMP3; Putative transmembrane protein C8orf57