Human/Mouse IRF2 Antibody

Catalog #: AF4529 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4529
AF4529-SP

Detection of Human/Mouse IRF2 by Western Blot.

1 Image

All IRF2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IRF2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse IRF2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse IRF2
Val151-Cys349
Accession # P23906

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse IRF2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse IRF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4529) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF2

Interferon Regulatory Factor 2 (IRF2) belongs to the interferon regulatory transcription factor family. IRF2 functions as a regulator of type I interferons by influencing cellular proliferation and immune response through transcriptional regulation. IRF2 competitively inhibits IRF1 gene activation, as well as, stimulating transcription on its own. Overexpression of IRF2 in NIH3T3 cells leads to transformation, whereas, loss of IRF2 expression in knock out mice leads to a variety of abnormalities including loss of NK cells due to increased apoptosis.

Long Name

Interferon Regulatory Factor 2

Entrez Gene IDs

3660 (Human); 16363 (Mouse); 290749 (Rat)

Alternate Names

DKFZp686F0244; interferon regulatory factor 2; IRF2; IRF-2