Human/Mouse LPP Antibody

Catalog #: MAB6927 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6927
MAB6927-SP

Detection of Human and Mouse LPP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse LPP Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human LPP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TRIP-4, -6, or -11 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 691121

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human LPP
Lys138-Gln261
Accession # Q93052

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse LPP by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human LPP Monoclonal Antibody (Catalog # MAB6927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LPP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LPP

Human LIM domain-containing preferred translocation partner in lipoma (LPP) is an 80 kDa intracellular protein that contains an N-terminal Pro-rich region (aa 41-370) followed by three tandem LIM domains (aa 414-603). It localizes to focal adhesion plaques where it regulates junction assembly and cytoskeleton remodeling through interactions with a-Actinin, VASP, SCRIB, Supervillin, and Palladin. In the nucleus, LPP coactivates the transcription factor PEA3 and protects telomeres from DNA damage. LPP is a frequent target of chromosomal translocations with HMG-A2 in lipoma. Within aa 138-261, human LPP shares 90% and 77% aa sequence identity with mouse and rat LPP, respectively.

Long Name

Lipoma-Preferred Partner

Entrez Gene IDs

4026 (Human); 210126 (Mouse); 288010 (Rat)

Alternate Names

DKFZp779O0231; FLJ30652; FLJ41512; LIM domain containing preferred translocation partner in lipoma; LIM domain-containing preferred translocation partner in lipoma; LIM protein; lipoma-preferred partner; LPP