Human/Mouse/Rat Follistatin-like 4/FSTL4 Antibody

Catalog #: AF5245 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5245
AF5245-SP

Detection of Human, Mouse, and Rat Follistatin‑like 4/FSTL4 by Western Blot.

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Human/Mouse/Rat Follistatin-like 4/FSTL4 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects recombinant mouse Follistatin-like 4/FSTL4 in direct ELISAs. Detects human, mouse, and rat Follistatin-like 4/FSTL4 in Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FSTL4 is observed, and less than 5% cross‑reactivity with rhFSTL5 and recombinant mouse FSTL1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Follistatin-like 4/FSTL4
Trp23-Val841
Accession # Q5STE3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Follistatin‑like 4/FSTL4 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Follistatin-like 4/FSTL4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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Follistatin-like 4/FSTL4 in Mouse Brain. Follistatin-like 4/FSTL4 was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons in the brainstem. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. This application has not been tested in human or rat samples

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-like 4/FSTL4

FSTL4 (Follistatin-like protein 4; also FSL4, SPIG1 and D/Bsp120I-1) is a likely secreted, 90 kDa (predicted) member of the follistatin gene family of TGF-beta superfamily inhibitors. It is widely expressed, being found in neurons (retinal ganglion and cerebellar Purkinje cells), cardiac muscle cells, smooth muscle cells and intestinal epithelium. Mature mouse FSTL4 is 819 amino acids (aa) in length (aa 23-841). It contains one kazal-like domain (aa 80-134), an EF-hand motif (aa 173-208), and two Ig-like domains (aa 250-336 and 340-425). There are two potential splice forms, one that shows an alternative start site at Met315, and a second that possesses a His residue substitution for aa 518-841. Full-length mature mouse FSTL4 shares 95% and 84% aa identity with rat and human FSTL4, respectively.

Entrez Gene IDs

23105 (Human); 320027 (Mouse); 303130 (Rat)

Alternate Names

Follistatin like 4; Follistatin-like 4; Follistatin-like protein 4; follistatin-related protein 4; FSL4; FSTL4; KIAA1061