Human/Mouse/Rat NDFIP1 Antibody

Catalog #: AF8246 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8246
AF8246-SP

Detection of Human, Mouse, and Rat NDFIP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat NDFIP1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat NDFIP1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NDFIP1
Ala10-Asp105
Accession # Q9BT67

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat NDFIP1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, SK-Mel-28 human malignant melanoma cell line, bEnd.3 mouse endothelioma cell line, Hepa 1-6 mouse hepatoma cell line, C6 rat glioma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NDFIP1 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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NDFIP1 in SH‑SY5Y Human Cell Line. NDFIP1 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NDFIP1

NDFIP1 (NEDD4 family-interacting protein 1 and NEDD4 WW domain-binding protein 5) is a 26 kDa widely expressed protein. NDFIP1 is thought to be part of a family of integral Golgi membrane proteins. It serves as an adaptor for several E3 ubiquitin ligases including NEDD4 and ITCH, and consequently modulates the stability of their targets. NDFIP1 is genetically linked to human allergic and autoimmune disease, but the cellular mechanism by which these proteins enable foreign and self-antigens to be tolerated is unresolved. Over aa10-105, human NDFIP1 is 95% identical to mouse and rat NDFIP1.

Long Name

Nedd4 Family Interacting Protein 1

Entrez Gene IDs

80762 (Human); 65113 (Mouse); 291609 (Rat)

Alternate Names

Breast cancer-associated protein SGA-1M; MGC10924; N4WBP5; N4WBP5Putative NF-kappa-B-activating protein 164; NDFIP1; Nedd4 family interacting protein 1; NEDD4 family-interacting protein 1; NEDD4 WW domain-binding protein 5; Putative MAPK-activating protein PM13; Putative NFKB and MAPK-activating protein