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Human/Mouse/Rat PGK1 Antibody

Catalog #: AF5455
2 Citations Datasheet / COA / SDS
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AF5455
AF5455-SP
Detection of Human/Mouse/Rat PGK1 by Western Blot.
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Citations (2)
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Human/Mouse/Rat PGK1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat PGK1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PGK1
Met1-Ile417
Accession # P00558
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PGK1 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PGK1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGK1 at approximately 43 to 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGK1

Phosphoglycerate kinase-1 (PGK1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1 alpha and is a potential biomarker and therapeutic target for cancer (4‑7).

References
  1. Beutler, E. (2002) Br. J. Haematol. 136:3.
  2. Flanagan, J.M. et al. (2006) Br. J. Haematol. 134:233.
  3. Svaasand, E.K. et al. (2007) Muscle Nerve. 36:579.
  4. Hwang, T.L. et al. (2006) Proteomics. 6:2259.
  5. Wang, J. et al. (2007) Cancer Res. 67:149.
  6. Zieker, D. et al. (2008) Cell Physiol. Biochem. 21:429.
  7. Jang, C.H. et al. (2008) Biosci. Biotechnol. Biochem. 72:1799.
Long Name
Phosphoglycerate Kinase-1
Entrez Gene IDs
5230 (Human); 18655 (Mouse); 24644 (Rat)
Alternate Names
Cell migration-inducing gene 10 protein; EC 2.7.2.3; MGC142128; MGC8947; MIG10; PGK1; PGKA; PGKAMGC117307; phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2; PRP2

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Citations for Human/Mouse/Rat PGK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A methyl-deficient diet fed to rats during the pre- and peri-conception periods of development modifies the hepatic proteome in the adult offspring
    Authors: Christopher A. Maloney, Susan M. Hay, Martin D. Reid, Gary Duncan, Fergus Nicol, Kevin D. Sinclair et al.
    Genes & Nutrition
  2. Metabolic alterations derived from absence of Two-Pore Channel 1 at cardiac level
    Authors: Francisca Lago
    J. Biosci., 2016-12-01;41(4):643-658.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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