Phospho‑ERK1/ERK2, (T202/Y204, T185/Y187) Fluorescein‑conjugated Antibody

Name: Phospho‑ERK1/ERK2, (T202/Y204, T185/Y187) Fluorescein‑conjugated Antibody
Brand: R&D Systems
SKU: IC1018F

Catalog #: IC1018F Datasheet / COA / SDS

Discontinued Product

IC1018F has been discontinued.

View all ERK1/ERK2 products.

Detection of ERK1/ERK2 in Jurkat Human Cell Line by Flow Cytometry.

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All ERK1/ERK2 Antibodies

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Phospho‑ERK1/ERK2, (T202/Y204, T185/Y187) Fluorescein‑conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing ERK1 T202/Y204 sites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of ERK1/ERK2 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of ERK1/ERK2 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line, resting (open histogram), or treated with PMA for 10 minutes (filled histogram) was stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1018F) or isotype control antibody (Catalog # IC105F, data not shown). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ERK1/ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Long Name

Extracellular Signal-regulated Kinase 1/2

Alternate Names

ERK1; ERK-1; ERK1/ERK2; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAPK 1; Microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; p44erk1; p44-ERK1; p44mapk; p44-MAPK; PRKM3