Human Myocilin Antibody

Catalog #: MAB34461 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB34461-100
MAB34461-SP

Detection of Myocilin in HEK293 cells by Flow Cytometry.

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All Myocilin Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Myocilin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Myocilin in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 297823

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Myocilin
Arg33-Met504
Accession # Q99972

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

Immersion fixed paraffin-embedded sections of Human Skeletal Muscle

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

HEK293 cells transfected with Human MYOC vs Irrelevant transfectant fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

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Detection of Myocilin in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human MYOC (filled histogram) vs Irrelevant transfectant cells (open histogram) were stained with Mouse Anti-Human Myocilin Monoclonal Antibody (Catalog # MAB34461), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Immunohistochemistry

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Detection of Myocilin in Human Skeletal Muscle. Myocilin was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human Myocilin Monoclonal Antibody (Catalog # MAB34461) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myocilin

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.

Entrez Gene IDs

4653 (Human)

Alternate Names

GLC1A; GLC1Amyocilin; GPOA; JOAG; JOAG1; MYOC; Myocilin; myocilin, trabecular meshwork inducible glucocorticoid response; TIGR; TIGRmutated trabecular meshwork-induced glucocorticoid response protein; Trabecular meshwork-induced glucocorticoid response protein