Human NEU-1/Sialidase-1 Antibody

Catalog #: MAB6860
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6860
MAB6860-SP

Detection of Human NEU‑1/Sialidase‑1 by Western Blot.

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Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NEU-1/Sialidase-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NEU-1/Sialidase-1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 688215

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NEU-1/Sialidase-1
Lys46-Leu415
Accession # Q99519

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human NEU-1/Sialidase-1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NEU‑1/Sialidase‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NEU-1/Sialidase-1 Monoclonal Antibody (Catalog # MAB6860) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NEU-1/Sialidase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NEU-1/Sialidase-1

Neuraminidase, also called NEU-1 or lysosomal sialidase, is a 45 kDa glycoprotein in the N-acetyl-a neuraminidase family. It is a member of a complex within the secretory lysosomes of melanosomes and hematopoietic cells that removes terminal sialic acids from glycosylated proteins and lipids. Mature human NEU-1 (aa 46‑415) shares 86.5% aa identity with mouse and rat NEU-1. A 225 aa splice variant shares aa 206‑415, but has an alternate N-terminus.

Long Name

N-acetyl-alpha-Neuraminidase 1

Entrez Gene IDs

4758 (Human); 18010 (Mouse); 24591 (Rat)

Alternate Names

Acetylneuraminyl hydrolase; EC 3.2.1.18; exo-alpha-sialidase; FLJ93471; G9 sialidase; Lysosomal sialidase; N-acetyl-alpha-neuraminidase 1; NANH; NEU; NEU1; NEU-1; SIAL1; sialidase 1 (lysosomal sialidase); Sialidase-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human NEU-1/Sialidase-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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