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Human NPY1R Antibody

Catalog #: MAB6400
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6400
MAB6400-SP
Detection of NPY1R in MCF‑7 Human Cell Line by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NPY1R Antibody Summary

Species Reactivity
Human
Specificity
Detects human NPY1R in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 556153
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human NPY1R
Met1-Ile384
Accession # P25929
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NPY1R antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of NPY1R in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line (Korner, M and J.C. Reubi (2007) Peptides28:419) was stained with Human NPY1R Monoclonal Antibody (Catalog # MAB6400, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPY1R

Neuropeptide Y receptor type 1 (NPY1R) is a 44 kDa (unglycosylated) member of the G protein coupled receptor 1 family. Human NPY1R is 384 amino acids (aa) in length and contains seven transmembrane regions and three potential sites for N-linked glycosylation. In addition, aa 338 is an S-palmitoyl cysteine lipid-binding site and aa 368 is a phosphoserine. Human NPY1R shares 94% and 93% aa sequence identity with mouse and rat NPY1R, respectively. Functionally, NPY1R is a receptor for neuropeptide Y and peptide YY.

Long Name
Neuropeptide Y Receptor Y1
Entrez Gene IDs
4886 (Human); 18166 (Mouse)
Alternate Names
neuropeptide Y receptor type 1; neuropeptide Y receptor Y1; NPY1R; NPY1-R; NPYR; NPYY1

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Citation for Human NPY1R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Ontogeny and Vulnerabilities of Drug-Tolerant Persisters in HER2+ Breast Cancer
    Authors: Chewei Anderson Chang, Jayu Jen, Shaowen Jiang, Azin Sayad, Arvind Singh Mer, Kevin R. Brown et al.
    Cancer Discovery

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