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Human PDGF-D Antibody

Catalog #: AF1159
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1159
AF1159-SP
Product Details
Citations (7)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF-D Antibody Summary

Species Reactivity
Human
Specificity
Detects human PDGF-D in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) PDGF-AA, rhPDGF-AB and rhPDGF-BB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PDGF-D
Asp253-Arg370
Accession # Q9GZP0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human PDGF-D
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human renal cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-D

PDGF-D, also named spinal cord-derived growth factor B (SCDGF-B) is a member of the PDGF/VEGF family.

Long Name
Platelet-derived Growth Factor D
Entrez Gene IDs
80310 (Human); 71785 (Mouse)
Alternate Names
IEGFMSTP036; Iris-expressed growth factor; PDGFD; PDGF-D; platelet derived growth factor D; SCDGF-BMGC26867; SCDGFBplatelet-derived growth factor D; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B

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Citations for Human PDGF-D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Overexpression of Platelet-derived Growth Factor-D as a Poor Prognosticator in Endometrial Cancer
    Authors: Jie Ding, Xiao-Mao Li, Sui-Ling Liu, Yu Zhang, Tian Li
    Asian Pacific Journal of Cancer Prevention
  2. Stromal reprogramming through dual PDGFRalpha/beta blockade boosts the efficacy of anti-PD-1 immunotherapy in fibrotic tumors
    Authors: T Akiyama, T Yasuda, T Uchihara, N Yasuda-Yos, BJY Tan, A Yonemura, T Semba, J Yamasaki, Y Komohara, K Ohnishi, F Wei, L Fu, J Zhang, F Kitamura, K Yamashita, K Eto, S Iwagami, H Tsukamoto, T Umemoto, M Masuda, O Nagano, Y Satou, H Saya, P Tan, H Baba, T Ishimoto
    Cancer Research, 2023-03-02;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. High expression of PDGFR-? in prostate cancer stroma is independently associated with clinical and biochemical prostate cancer recurrence
    Authors: Y Nordby, E Richardsen, M Rakaee, N Ness, T Donnem, HR Patel, LT Busund, RM Bremnes, S Andersen
    Sci Rep, 2017-02-24;7(0):43378.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Dynamic regulation of platelet-derived growth factor D (PDGF-D) activity and extracellular spatial distribution by matriptase-mediated proteolysis.
    Authors: Huang W, Kim H
    J Biol Chem, 2015-02-12;290(14):9162-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression.
    Authors: Wagsater D, Zhu C, Bjorck HM, Eriksson P
    Atherosclerosis, 2008-05-22;202(2):415-23.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: IHC-Fr, Neutralization, Western Blot
  6. Expression of platelet-derived growth factors C and D in the synovial membrane of patients with rheumatoid arthritis and osteoarthritis.
    Authors: Pohlers D, Huber R, Ukena B, Kinne RW
    Arthritis Rheum., 2006-03-01;54(3):788-94.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  7. Platelet-Derived Growth Factors in Non-GIST Soft-Tissue Sarcomas Identify a Subgroup of Patients with Wide Resection Margins and Poor Disease-Specific Survival
    Authors: Thomas Karsten Kilvaer, Andrej Valkov, Sveinung W. Sorbye, Tom Donnem, Eivind Smeland, Roy Martin Bremnes et al.
    Sarcoma

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