Human SLC5A8/SMCT1 Antibody

Catalog #: MAB8398 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8398
MAB8398-SP

Detection of SLC5A8/SMCT1 in MCF‑7 Human Cell Line by Flow Cytometry.

2 Images

All SLC5A8/SMCT1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLC5A8/SMCT1 Antibody Summary

Species Reactivity

Human

Specificity

Detects HEK293 human embryonic kidney cell line transfected with human SLC5A8/SMCT1 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Source

Monoclonal Mouse IgG₁ Clone # 903502

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SLC5A8/SMCT1
Met1-Leu610
Accession # NP_666018

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of SLC5A8/SMCT1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Flow Cytometry

View Larger

Detection of SLC5A8/SMCT1 in HEK293 Human Cell Line Transfected with Human SLC5A8/SMCT1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human SLC5A8/SMCT1 and eGFP was stained with either (A) Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC5A8/SMCT1

SLC5A8/SMCT1 (Solute Carrier family 5, member 8) is a member of the solute carrier family of multi-pass membrane proteins. SLC5A8 has been shown to transport iodide by a passive mechanism. It also transports monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism. SLC5A8 nuclear translocation and loss of expression are associated with poor outcome in pancreatic ductal adenocarcinoma (1). It may be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract.

References

Helm, J. et al. (2012) Pancreas 41:904.

Long Name

Solute Carrier Family 5 Iodide Transporter), Member 8

Entrez Gene IDs

160728 (Human); 216225 (Mouse); 500820 (Rat)

Alternate Names

AIT; AITSolute carrier family 5 member 8; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; MGC125354; SLC5A8; SMCT; SMCTSMCT1; Sodium iodide-related cotransporter; sodium-coupled monocarboxylate transporter 1; solute carrier family 5 (iodide transporter), member 8