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Human TBX6 Antibody

Catalog #: AF4744
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4744
AF4744-SP
Detection of Human TBX6 by Western Blot.
3 Images
Product Details
Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TBX6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TBX6 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TBX6
Met1-Arg280
Accession # O95947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
0.2-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TBX6 antibody by Western Blot. View Larger

Detection of Human TBX6 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line and HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TBX6 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry TBX6 antibody in Embryonic Mouse Mesoderm by Immunohistochemistry (IHC-Fr). View Larger

TBX6 in Embryonic Mouse Mesoderm. TBX6 was detected in immersion fixed frozen sections of embryonic mouse mesoderm (E9.5) using 10 µg/mL Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunocytochemistry TBX6 antibody in JOY6 Human iPS Cells by Immunocytochemistry (ICC). View Larger

TBX6 in JOY6 Human iPS Cells. TBX6 was detected in immersion fixed JOY6 human induced pluripotent stem cells undifferentiated (right panel) and differentiated into mesoderm (left panel) using Goat Anti-Human TBX6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4744) at 0.2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei in mesoderm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX6

TBX6 (T-box protein 6) is a 47 kDa member of the T-box family of transcriptional regulators. It is expressed during embryogenesis, plays a role in presomitic mesoderm development, and promotes delta-like 1 transcription. Human TBX6 is 436 amino acids (aa) in length. It contains one T‑box DNA-binding domain (aa 90‑277). There is one alternate splice form that shows a 15 aa substitution for aa 281‑436. Over aa 1‑280, human TBX6 shows 94% and 90% aa identity with canine and mouse TBX6, respectively.

Long Name
T-box Transcription Factor 6 [Ulnar Mammary Syndrome]
Entrez Gene IDs
6911 (Human); 21389 (Mouse); 365371 (Rat)
Alternate Names
DFNB67; T-box 6; T-box protein 6; T-box transcription factor TBX6; TBX6

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Citations for Human TBX6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C
    Authors: Daya, NM;Döring, K;Zhuge, H;Volke, L;Stab, V;Dietz, J;Athamneh, M;Roos, A;Zaehres, H;Güttsches, AK;Mavrommatis, L;Vorgerd, M;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Generation of two human iPSC lines (HIMRi002-A and HIMRi003-A) derived from Caveolinopathy patients with rippling muscle disease
    Authors: Boeing, A;Mavrommatis, L;Daya, NM;Zhuge, H;Volke, L;Kocabas, A;Kneifel, M;Athamneh, M;Krause, K;Südkamp, N;Döring, K;Theiss, C;Roos, A;Zaehres, H;Güttsches, AK;Vorgerd, M;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative
    Authors: Balducci, V;Scardigli, F;Harakalova, M;Peter van Tintelen, J;Doevendans, PA;Costa, KD;Turnbull, IC;P G Sluijter, J;Stillitano, F;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells
    Authors: Marina Gattiglio, Michelle Protzek, Christian Schröter
    Biology Open
  5. A gastruloid model of the interaction between embryonic and extra-embryonic cell types
    Authors: NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau
    Journal of tissue engineering, 2022-06-11;13(0):2041731422110.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  6. Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration
    Authors: MK Shin, JS Bang, JE Lee, HD Tran, G Park, DR Lee, J Jo
    International Journal of Molecular Sciences, 2022-05-04;23(9):.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  7. Statistically derived geometrical landscapes capture principles of decision-making dynamics during cell fate transitions
    Authors: Meritxell Sáez, Robert Blassberg, Elena Camacho-Aguilar, Eric D. Siggia, David A. Rand, James Briscoe
    Cell Systems
  8. A Tgfbr1/Snai1-dependent developmental module at the core of vertebrate axial elongation
    Authors: André Dias, Anastasiia Lozovska, Filip J Wymeersch, Ana Nóvoa, Anahi Binagui-Casas, Daniel Sobral et al.
    eLife
  9. Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
    Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
    Cell Rep, 2020-04-07;31(1):107471.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. A dorsal-ventral gradient of Wnt3a/ beta -catenin signals controls mouse hindgut extension and colon formation
    Authors: Robert J. Garriock, Ravindra B. Chalamalasetty, JianJian Zhu, Mark W. Kennedy, Amit Kumar, Susan Mackem et al.
    Development
  11. Generation of eight human induced pluripotent stem cell lines from Parkinson's disease patients carrying familial mutations
    Authors: M Chen, M Maimaitili, SH Buchholdt, UB Jensen, F Febbraro, M Denham
    Stem Cell Res, 2019-11-13;42(0):101657.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells
    Authors: Li-Fang Chu, Daniel Mamott, Zijian Ni, Rhonda Bacher, Cathy Liu, Scott Swanson et al.
    Cell Reports
  13. Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions
    Authors: Aida Rodrigo Rodrigo Albors, Pamela A. Halley, Kate G. Storey
    Development
  14. Human axial progenitors generate trunk neural crest cells in vitro
    Authors: Thomas JR Frith, Ilaria Granata, Matthew Wind, Erin Stout, Oliver Thompson, Katrin Neumann et al.
    eLife
  15. Assessing the bipotency of in vitro-derived neuromesodermal progenitors
    Authors: Anestis Tsakiridis, Valerie Wilson
    F1000Research
  16. Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
    Authors: Garriock R, Chalamalasetty R, Kennedy M, Canizales L, Lewandoski M, Yamaguchi T
    Development, 2015-05-01;142(9):1628-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
    Authors: Gouti, Mina, Tsakiridis, Anestis, Wymeersch, Filip J, Huang, Yali, Kleinjung, Jens, Wilson, Valerie, Briscoe, James
    PLoS Biol, 2014-08-26;12(8):e1001937.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  18. Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.
    Authors: Cheung C, Bernardo AS, Trotter MW
    Nat. Biotechnol., 2012-01-15;30(2):165-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Cooperation between ETS transcription factor ETV1 and histone demethylase JMJD1A in colorectal cancer.
    Authors: Oh, S, Song, H Et al.
    Int J Oncol

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