Human TfR2 Antibody

Catalog #: MAB31202 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB31202-100
MAB31202-SP

Detection of Human TfR2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TfR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TfR2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 353810

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TfR2
Arg105-Phe801
Accession # Q9UP52

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

K562 human chronic myelogenous leukemia cell line

Flow Cytometry

0.25 µg/10⁶ cells

K562 human myelogenous leukemia cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TfR2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TfR2 Monoclonal Antibody (Catalog # MAB31202) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TfR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry

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Detection of TfR2 in K562 Human Cell Line by Flow Cytometry. K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human TfR2 Monoclonal Antibody (Catalog # MAB31202, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TfR2

Transferrin Receptor 2 (TfR2) is an iron-binding protein that is homologous to the transferrin receptor. Lack of active TfR2 has been associated with a rare form of hemochromatosis. The TfR2 alpha isoform is a type 2 transmembrane protein that is expressed in the liver and modulates hepcidin production in response to iron. It forms a 215 kDa disulfide-linked homodimer that is presumably glycated. The TfR2 beta isoform lacks the intracellular, transmembrane and part of the extracellular regions and is expressed ubiquitously. The extracellular portion of human TfR2 shares 86% aa identity with mouse.

Long Name

Transferrin Receptor 2

Entrez Gene IDs

7036 (Human); 50765 (Mouse)

Alternate Names

HFE3; HFE3transferrin receptor protein 2; MGC126368; TfR2; TFRC2; transferrin receptor 2