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Human TM4SF4 APC-conjugated Antibody

Catalog #: FAB7998A
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FAB7998A
Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry.
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Human TM4SF4 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TM4SF4 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 832441
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TM4SF4
Accession # P48230
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TM4SF4 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TM4SF4 APC-conjugated Monoclonal Antibody (Catalog # FAB7998A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TM4SF4

TM4SF4 (Transmembrane 4 L6 Family Member 4), also known as IL-TMP (Intestine and Liver Tetraspanin Membrane Protein), is a member of the L6 tetraspanin family of molecules. It is expressed at highest levels on non-proliferating villus-associated intestinal epithelia and periportal hepatocytes, with increased expression in hepatocellular carcinomas. It is thought to mediate proliferation and adhesion. Its molecular size is reported at approximately 21 kDa for the unglycosylated form and 25-40 kDa for various glycosylated forms.  The 202 amino acid (aa) human TM4SF4 contains four transmembrane domains and two potential N-glycosylation sites. It shares 88% aa sequence identity with mouse and rat TM4SF4.

Long Name
Transmembrane 4 L Six Family Member 4
Entrez Gene IDs
7104 (Human); 229302 (Mouse); 116467 (Rat)
Alternate Names
FLJ31015; ILTMP; il-TMP; ILTMPintestinal and liver (il) tetraspan membrane protein; Intestine and liver tetraspan membrane protein; TM4SF4; transmembrane 4 L six family member 4; transmembrane 4 L6 family member 4; transmembrane 4 superfamily member 4

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Citation for Human TM4SF4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human gain-of-function variants in HNF1A confer protection from diabetes but independently increase hepatic secretion of atherogenic lipoproteins
    Authors: Natalie DeForest, Babu Kavitha, Siqi Hu, Roi Isaac, Lynne Krohn, Minxian Wang et al.
    Cell Genomics

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