Human TMPRSS9 Antibody

Catalog #: MAB56881 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB56881-100
MAB56881-SP

Detection of Human TMPRSS9 in HEK293 cell line transfected with human TMPRSS9 by Flow Cytometry.

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Human TMPRSS9 Antibody Summary

Species Reactivity

Human

Specificity

Detects TMPRSS9 in direct Elisa

Source

Monoclonal Mouse IgG₁ Clone # 1042526

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TMPRSS9
Glu190-Gln490
Accession # Q7Z410

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

25 µg/mL

HEK293 human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Human TMPRSS9 in HEK293 cell line transfected with human TMPRSS9 by Flow Cytometry. HEK293 cells were transfected with human TMPRSS9 (A) or an irrelevant protein (B), and stained with Mouse Anti-Human TMPRSS9 Monoclonal Antibody (Catalog # MAB56881), followed by staining with Allophycocyanin Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol. Mouse IgG1 is the Isotype Control (Not shown; MAB002).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMPRSS9

TMPRSS9 (Transmembrane protease, Serine 9; also Polyserine protease 1) is a 116-120 kDa (predicted) member of the TTSP family of membrane-bound serine proteases. It is expressed in fetal tissue, and converts pro-uPA into its active form. Human TMPRSS9 is a 1059 type II transmembrane (TM) protein. It has a 29 aa N-terminal cytoplasmic region and a 1008 aa extracellular domain (ECD) (aa 51-1059). The ECD contains one LDLR region (aa 153-190), followed by three Peptidase S1 domains (aa 203-436, 504-736, and 827-1058), the first two of which are catalytically active. Proteolytic cleavage can generate a soluble 34 kDa Serase 1 (aa 203-503), a 35 kDa Serase 2 (aa 504-826) and an inactive 25 kDa Serase 3 (aa 827-1059). Alternatively, truncated, TM forms of TMPRSS9 such as a 56 kDa TM isotype containing only the Serase 1 domain, or a 91 kDa (predicted) form containing the Serase 1, 2 and 3 domains may exist. Two potential splice forms are reported. One shows an insertion of 34 aa after Lys90 accompanied by a seven aa substitution for aa 491-1059. A second isoform shows a 34 aa substitution for aa 822-1059. Over aa 190-490, human TMPRSS9 shares 79% aa identity with mouse TMPRSS9.

Long Name

Transmembrane protease, serine 9

Entrez Gene IDs

360200 (Human); 432478 (Mouse); 314636 (Rat)

Alternate Names

EC 3.4.21; EC 3.4.21.4; Polyserase 1; Polyserase-1; Polyserase-I; Polyserine Protease 1; TMPRSS9; Transmembrane Protease Serine 9; Transmembrane Protease, Serine 9; Transmembrane Serine Protease 9