K 114

Biological Activity

Used for: amyloid fibril detection.

Application: fluorescence microscopy, confocal microscopy.

Properties and Photophysical Data: K 114 exhibits minimal fluorescence in aqueous buffers and fluoresces brightly in the presence of Aβ (1-40), α-synuclein and tau in situ. K 114 fluorescence is ph-dependent. Excitation and emission maxima (λ) are 370nm and 450 nm, respectively, at pH 8.5; excitation and emission maxima (λ) are 395nm and 520 nm, respectively, at pH 10.5.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. A comparison of amyloid fibrillogenesis using the novel fluorescent compound K114.
   Crystal et al.
   J.Neurochem., 2003;86:1359
2. Mechanism of Aβ(1-40) fibril-induced fluorescence of (trans-trans)-1-bromo-2,5-bis(4-hydroxystyryl)benene (K114).
   LeVine
   Biochemistry, 2005;44:15937

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