LOX Overexpression Lysate
Novus Biologicals | Catalog # NBL1-12640
![Western Blot: LOX Overexpression Lysate [NBL1-12640]](https://resources.rndsystems.com/images/products/LOX-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12640-img0003.jpg "Western Blot: LOX Overexpression Lysate [NBL1-12640]")
Key Product Details
Species
Applications
Product Summary for LOX Overexpression Lysate
LOX Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC213323
Accession#: NM_002317
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Scientific Data Images for LOX Overexpression Lysate
Western Blot: LOX Overexpression Lysate [NBL1-12640]
Western Blot: LOX Overexpression Lysate (Adult Normal) [NBL1-12640] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for LOX.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: Lysyl Oxidase/LOX
Long Name
Alternate Names
Gene Symbol
Additional Lysyl Oxidase/LOX Products
Product Documents for LOX Overexpression Lysate
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LOX Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for LOX Overexpression Lysate
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Q: I had a question about your product NBL1-12640, which is the LOX lysate. I was wondering if the LOX in there was active or is it an inactive protein? Can we activate it with some other agent like BAPN?
A: The LOX is inactive. The lab said that this lysate has not been tested for activity, nor have they attempted to activate it with some other agent like BAPN.
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Q: Why doesn't the lox lysate express active lox (32 kDa)?
A: The reason why this lysate does not express the active band at a lower molecular weight is that the full length sequence is used. The sequence is also tagged with DDK tag and anti DDK tag antibody is used for detection on Western Blot, which was picked up at the higher molecular weight. It is associated with this sequence that is expressed approximately 47 kDa (UniProt P28300).
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Q: I had a question about your product NBL1-12640, which is the LOX lysate. I was wondering if the LOX in there was active or is it an inactive protein? Can we activate it with some other agent like BAPN?
A: The LOX is inactive. The lab said that this lysate has not been tested for activity, nor have they attempted to activate it with some other agent like BAPN.
-
Q: Why doesn't the lox lysate express active lox (32 kDa)?
A: The reason why this lysate does not express the active band at a lower molecular weight is that the full length sequence is used. The sequence is also tagged with DDK tag and anti DDK tag antibody is used for detection on Western Blot, which was picked up at the higher molecular weight. It is associated with this sequence that is expressed approximately 47 kDa (UniProt P28300).