Mouse CD4 Biotinylated Antibody

Catalog #: BAF554 Datasheet / COA / SDS

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BAF554

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Mouse CD4 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CD4 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human CD4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD4
Lys27-Thr394
Accession # P06332

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse CD4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4 (Cluster of Differentiation #4; also L3T4) is a 55 kDa member of the Ig Superfamily of molecules. It is expressed on a variety of hematopoietic cell types, including immature thymocytes, a number of variable phenotype single positive T cells (i.e.- CD25⁺; CD39⁺; Th1, 2 and 17 type cells), stimulated CD8⁺ T cells, pluripotent bone marrow stem cells, and NKT cells. Although CD4 is a standard phenotype marker, functionally, the molecule is known to bind to APC MHC class II, and to associate with adjacent CD3 molecules to link p56^lck with the lymphocyte TCR:CD3 complex. Mature mouse CD4 is a 431 amino acid (aa) type I transmembrane glycoprotein. It contains a 368 aa extracellular region (aa 27-394) plus a 40 aa cytoplasmic domain (aa 418-457). The extracellular region contains one V-type (aa 27-128) and three C2-type Ig-like domains (aa 129-374). There is one alternative start site at Met241 that is utilized in brain. Over aa 27-394, mouse CD4 shares 53% and 72% aa identity with human and rat CD4, respectively.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4