Mouse Complement Component C3aR Antibody

Catalog #: MAB10417 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10417-100
MAB10417-SP

Detection of Complement Component C3aR in HEK293 Human Cell Line Transfected with Mouse Complement Component C3aRand eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Complement Component C3aR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse C3aR in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 620613

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived transfected with mouse C3aR
Accession # O09047

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Complement Component C3aR antibody in HEK293 Human Cell Line Transfected with Mouse Complement Component C3aRand eGFP antibody by Flow Cytometry.

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Detection of Complement Component C3aR in HEK293 Human Cell Line Transfected with Mouse Complement Component C3aRand eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Complement Component C3aR and eGFP was stained with (A) Rat Anti-Mouse Complement Component C3aR Monoclonal Antibody (Catalog # MAB10417) or (B) Rat IgG2a isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C3aR

Complement Component 3a receptor (C3a R) is an ~90 kDa G-protein coupled 7-transmembrane sulfated glycoprotein expressed on myeloid peripheral blood leukocytes, and on activated lymphocytes, endothelial and internal organ epithelial cells. Engagement of C3a R by C3a activates mast cells and neutrophils, triggering degranulation in airways during asthmatic allergen challenges. It assists in Th2-type inflammatory reactions and stimulates leukocyte chemotaxis. Human C3a R extracellular regions share 50% and 42% amino acid identity with mouse and rat C3a R, respectively.

Long Name

Complement Component C3a Receptor

Entrez Gene IDs

719 (Human); 12267 (Mouse)

Alternate Names

AZ3B; AZ3BC3a anaphylatoxin chemotactic receptor; C3AR; C3a-R; C3AR1; C3ARcomplement component 3 receptor 1; C3R1; complement component 3a receptor 1; Complement Component C3a R; Complement Component C3aR; HNFAG09