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Mouse Dkk-3 Biotinylated Antibody

Catalog #: BAF948
1 Citation Datasheet / COA / SDS
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BAF948
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Citations (1)
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Mouse Dkk-3 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Dkk-3 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dkk-3 is observed and less than 1% cross-reactivity with rhDkk-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dkk-3
Met1-Ile349
Accession # Q9QUN9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Dkk‑3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dkk-3

Dkk-3 (Dickkopf-related protein 3; also REIC) is a 65-70 kDa secreted glycoprotein member of the Dickkopf family of molecules. It is expressed by multiple cell types, including neurons, endothelial cells, keratinocytes and zona glomerulosa cells of the adrenal. Dkk-3 is reported to be a positive regulator of Wnt signaling. It is suggested to bind exclusively to Wnt-inhibiting Kremen proteins, thereby blocking their expression on the cell surface. This makes LRP available for Wnt binding and signaling. Dkk-3 is also reported to induce the development of DC from monocytes. Mature mouse Dkk-3 is 328 amino acids (aa) in length (aa 22-349). It contains one coiled-coil region (aa 39-84), two consecutive Dkk-type Cys-rich regions (aa 147-195 and 208-284), and one phosphoserine site at Ser298. Over aa 22-349, mouse shares 83% and 92% aa identity with human and rat Dkk-3, respectively.

Long Name
Dickkopf-3
Entrez Gene IDs
27122 (Human); 50781 (Mouse)
Alternate Names
dickkopf (Xenopus laevis) homolog 3; dickkopf 3; dickkopf homolog 3 (Xenopus laevis); Dickkopf-3; dickkopf-related protein 3; Dkk3; Dkk-3; hDkk-3; regulated in glioma; REIC; REICdkk-3; RIG; RIG-like 5-6; RIG-like 7-1

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Citation for Mouse Dkk-3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Self-Antigen Presentation by Keratinocytes in the Inflamed Adult Skin Modulates T-Cell Auto-Reactivity.
    Authors: Meister M, Tounsi A, Gaffal E, Bald T, Papatriantafyllou M, Ludwig J, Pougialis G, Bestvater F, Klotz L, Moldenhauer G, Tuting T, Hammerling G, Arnold B, Oelert T
    J Invest Dermatol, 2015-04-02;135(8):1996-2004.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)

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