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Mouse ESAM Antibody

Catalog #: AF2827
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2827
AF2827-SP
Detection of ESAM in bEnd.3 cells by Flow Cytometry
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse ESAM Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ESAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human ESAM is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ESAM
Gln30-Ser248 (predicted)
Accession # Q925F2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse ESAM
Flow Cytometry
0.25 µg/106 cells
bEnd.3 mouse endothelioma cell line
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of mouse kidney and liver
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of ESAM in bEnd.3 cells by Flow Cytometry bEnd.3 cells were stained with Goat Anti-Mouse ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2827, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry View Larger

Detection of ESAM in Mouse Kidney. ESAM was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # af2827) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of vascular endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry View Larger

Detection of ESAM in Mouse Liver. ESAM was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # af2827) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of vascular endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ESAM

ESAM is a 55 kDa type I transmembrane glycoprotein belonging to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members are CXADR, CLMP, JAM-A-C, CD2, A33, and BT-IgSF. The extracellular region of ESAM contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion. Mouse ESAM extracellular domain shares 69% amino acid sequence identity with the corresponding region of human ESAM. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes and can be found associated with cell-to-cell junctions.

Long Name
Endothelial Cell Adhesion Molecule
Entrez Gene IDs
90952 (Human); 69524 (Mouse)
Alternate Names
2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; ESAM; HUEL (C4orf1)-interacting protein; LP4791 protein; W117m

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Citations for Mouse ESAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Claudin5 protects the peripheral endothelial barrier in an organ and vessel-type-specific manner
    Authors: Mark Richards, Emmanuel Nwadozi, Sagnik Pal, Pernilla Martinsson, Mika Kaakinen, Marleen Gloger et al.
    eLife
  2. A Prox1 enhancer represses haematopoiesis in the lymphatic vasculature
    Authors: J Kazenwadel, P Venugopal, A Oszmiana, J Toubia, L Arriola-Ma, V Panara, SG Piltz, C Brown, W Ma, AW Schreiber, K Koltowska, S Taoudi, PQ Thomas, HS Scott, NL Harvey
    Nature, 2023-01-25;614(7947):343-348.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. A RORgammat+ cell instructs gut microbiota-specific Treg cell differentiation
    Authors: R Kedmi, TA Najar, KR Mesa, A Grayson, L Kroehling, Y Hao, S Hao, M Pokrovskii, M Xu, J Talbot, J Wang, J Germino, CA Lareau, AT Satpathy, MS Anderson, TM Laufer, I Aifantis, JM Bartleson, PM Allen, H Paidassi, JM Gardner, M Stoeckius, DR Littman
    Nature, 2022-09-07;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: CITE-seq

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