Mouse FoxD3 Antibody

Catalog #: MAB5090 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5090
MAB5090-SP

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Mouse FoxD3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FoxD3 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FoxD3 is observed.

Source

Monoclonal Rat IgG_2A Clone # 800602

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse FoxD3
Met1-Val130
Accession # Q61060

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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FoxD3 in D3 Mouse Stem Cell Line. FoxD3 was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse FoxD3 Monoclonal Antibody (Catalog # MAB5090) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxD3

FoxD3 (forkhead box protein D3; also HNF3, HFH2 and Genesis) is a 50 kDa member of the winged helix transcription factor gene family. It is expressed in the epiblast, migrating neural crest, and motoneurons plus interneuron progenitors of the developing spinal cord. Mouse FoxD3 is 465 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 131-208) plus an Ala-rich region (aa 248-464) that contains a Gly-rich motif (aa 369-399). There appears to be one splice form that shows a four aa insert after Gly261. Over aa 1-130, mouse FoxD3 shares 97% and 79% aa identity with rat and human FoxD3, respectively.

Long Name

Forkhead Box D3

Entrez Gene IDs

27022 (Human); 15221 (Mouse)

Alternate Names

AIS1; CWH3; forkhead box D3; forkhead box protein D3; FoxD3; Genesis; HFH2; HNF3/FH transcription factor genesis