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Mouse Granzyme B Antibody

Catalog #: AF1865
25 Citations 1 Review Datasheet / COA / SDS
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AF1865
AF1865-SP
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Granzyme B in Mouse Splenocytes.
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Product Details
Citations (25)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Granzyme B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Granzyme B in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
Lys17-Ser247
Accession # P04187
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Granzyme B (Catalog # 1865-SE)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Granzyme B (Catalog # 1865-SE), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Mouse Granzyme B Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Granzyme B Biotinylated Antibody (Catalog # BAF1865)

Standard: Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)

Neutralization
Measured by its ability to neutralize Recombinant Mouse Granzyme B (0.5 µg/mL, Catalog # 1865-SE) cleavage of the fluorogenic peptide substrate Boc-AAD-SBzl (100 µM). The Neutralization Dose (ND50) is typically 3.5 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Granzyme B antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Granzyme B in Mouse Splenocytes. Granzyme B was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Granzyme B antibody in Mouse Spleen Tissue by Immunohistochemistry (IHC-P). View Larger

Granzyme B in Mouse Spleen Tissue. Granzyme B was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Mouse Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1865) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

References
  1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
  4. Lobe, C.G. et al. (1986) Science 232:858.
  5. Brunet, J.F. et al. (1986) Nature 322:268.
Entrez Gene IDs
3002 (Human); 14939 (Mouse)
Alternate Names
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT

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Citations for Mouse Granzyme B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation
    Authors: Hung-Hsi Chen, Hsin-I Yu, Rudy Rudy, Sim-Lin Lim, Yi-Fen Chen, Shu-Hsing Wu et al.
    iScience
  2. Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1
    Authors: Cha JH, Yang WH, Xia W et al.
    Mol. Cell
  3. Impaired regulatory function of granzyme B-producing B cells against T cell inflammatory responses in lupus mice
    Authors: Jimeng Xue, Liling Xu, Hua Zhong, Mingxin Bai, Xin Li, Ranran Yao et al.
    Lupus Science & Medicine
  4. Cationic-nanogel nasal vaccine containing the ectodomain of RSV-small hydrophobic protein induces protective immunity in rodents
    Authors: Shingo Umemoto, Rika Nakahashi-Ouchida, Yoshikazu Yuki, Shiho Kurokawa, Tomonori Machita, Yohei Uchida et al.
    npj Vaccines
  5. BMI1 Inhibition Eliminates Residual Cancer Stem Cells after PD1 Blockade and Activates Antitumor Immunity to Prevent Metastasis and Relapse
    Authors: L Jia, W Zhang, CY Wang
    Cell Stem Cell, 2020-07-21;27(2):238-253.e6.
  6. circFAT1 Promotes Cancer Stemness and Immune Evasion by Promoting STAT3 Activation
    Authors: Lingfei Jia, Yilun Wang, Cun‐Yu Wang
    Advanced Science
  7. Juxtacrine signaling inhibits antitumor immunity by upregulating PD-L1 expression
    Authors: WH Yang, JH Cha, W Xia, HH Lee, LC Chan, YN Wang, JL Hsu, G Ren, MC Hung
    Cancer Res., 2018-05-22;0(0):.
  8. Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stress
    Authors: Wuchang Zhang, Wei Liu, Lingfei Jia, Demeng Chen, Insoon Chang, Michael Lake et al.
    Molecular Cell
  9. Novel Combinatorial MicroRNA-Binding Sites in AAV Vectors Synergistically Diminish Antigen Presentation and Transgene Immunity for Efficient and Stable Transduction
    Authors: Manish Muhuri, Wei Zhan, Yukiko Maeda, Jia Li, Anoushka Lotun, Jennifer Chen et al.
    Frontiers in Immunology
  10. Immunotherapy-induced cytotoxic T follicular helper cells reduce numbers of retrovirus-infected reservoir cells in B cell follicles
    Authors: Malyshkina, A;Bayer, W;Podschwadt, P;Otto, L;Karakoese, Z;Sutter, K;Bruderek, K;Wang, B;Lavender, KJ;Santiago, ML;Leipe, PM;Elsner, C;Esser, S;Brandau, S;Gunzer, M;Dittmer, U;
    PLoS pathogens
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Preclinical PET Imaging of Granzyme B Shows Promotion of Immunological Response Following Combination Paclitaxel and Immune Checkpoint Inhibition in Triple Negative Breast Cancer
    Authors: TS Napier, CL Hunter, PN Song, BM Larimer, AG Sorace
    Pharmaceutics, 2022-02-18;14(2):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. ST3GAL2 knock-down decreases tumoral character of colorectal cancer cells in vitro and in vivo
    Authors: M Deschuyter, DY Leger, A Verboom, A Chaunavel, A Maftah, JM Petit
    American journal of cancer research, 2022-01-15;12(1):280-302.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. CD8+ tissue-resident memory T cells promote liver fibrosis resolution by inducing apoptosis of hepatic stellate cells
    Authors: Y Koda, T Teratani, PS Chu, Y Hagihara, Y Mikami, Y Harada, H Tsujikawa, K Miyamoto, T Suzuki, N Taniki, T Sujino, M Sakamoto, T Kanai, N Nakamoto
    Nature Communications, 2021-07-22;12(1):4474.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. IL-15, gluten and HLA-DQ8 drive tissue destruction in coeliac disease
    Authors: V Abadie, SM Kim, T Lejeune, BA Palanski, JD Ernest, O Tastet, J Voisine, V Discepolo, EV Marietta, MBF Hawash, C Ciszewski, R Bouziat, K Panigrahi, I Horwath, MA Zurenski, I Lawrence, A Dumaine, V Yotova, JC Grenier, JA Murray, C Khosla, LB Barreiro, B Jabri
    Nature, 2020-02-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. IL-6/JAK1 pathway drives PD-L1 Y112 phosphorylation to promote cancer immune evasion
    Authors: LC Chan, CW Li, W Xia, JM Hsu, HH Lee, JH Cha, HL Wang, WH Yang, EY Yen, WC Chang, Z Zha, SO Lim, YJ Lai, C Liu, J Liu, Q Dong, Y Yang, L Sun, Y Wei, L Nie, JL Hsu, H Li, Q Ye, MM Hassan, HM Amin, AO Kaseb, X Lin, SC Wang, MC Hung
    J. Clin. Invest., 2019-07-15;130(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. PARP inhibitor efficacy depends on CD8+ T cell recruitment via intratumoral STING pathway activation in BRCA-deficient models of triple-negative breast cancer
    Authors: C Pantelidou, O Sonzogni, M de Oliveir, AK Mehta, A Kothari, D Wang, T Visal, MK Li, J Pinto, JA Castrillon, EM Cheney, P Bouwman, J Jonkers, S Rottenberg, JL Guerriero, GM Wulf, GI Shapiro
    Cancer Discov, 2019-04-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Novel Recombinant DNA Vaccine Candidates for Human Respiratory Syncytial Virus: Preclinical Evaluation of Immunogenicity and Protection Efficiency
    Authors: MA Farrag, HM Amer, P Öhlschläge, ME Hamad, FN Almajhdi
    Hum Vaccin Immunother, 2017-03-08;0(0):0.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  18. Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
    Authors: Whitehead M, Ohlschlager P, Almajhdi F, Alloza L, Marzabal P, Meyers A, Hitzeroth I, Rybicki E
    BMC Cancer, 2014-05-24;14(0):367.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  19. Endogenous retinoids in the pathogenesis of alopecia areata.
    Authors: Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H
    J Invest Dermatol, 2012-09-27;133(2):334-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Serine protease inhibitor 6 protects cytotoxic T cells from self-inflicted injury by ensuring the integrity of cytotoxic granules.
    Authors: Zhang M, Park SM, Wang Y, Shah R, Liu N, Murmann AE, Wang CR, Peter ME, Ashton-Rickardt PG
    Immunity, 2006-04-01;24(4):451-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. The let-7 family of microRNAs suppresses immune evasion in head and neck squamous cell carcinoma by promoting PD-L1 degradation
    Authors: Dan Yu, Xueshibojie Liu, Guanghong Han, Yan Liu, Xue Zhao, Di Wang et al.
    Cell Communication and Signaling
  22. Immune Remodeling of the Extracellular Matrix Drives Loss of Cancer Stem Cells and Tumor Rejection.
    Authors: Pires, A, Greenshields-Watson, A Et al.
    Cancer Immunol Res
  23. Design of a Highly Effective Therapeutic HPV16 E6/E7-Specific DNA Vaccine: Optimization by Different Ways of Sequence Rearrangements (Shuffling)
    Authors: Fahad N. Almajhdi, Tilo Senger, Haitham M. Amer, Lutz Gissmann, Peter Öhlschläger
    PLoS ONE
  24. RNF144A deficiency promotes PD-L1 protein stabilization and carcinogen-induced bladder tumorigenesis
    Authors: Shiuh-Rong Ho, Yu-Cheng Lee, Michael M. Ittmann, Fang-Tsyr Lin, Keith Syson Chan, Weei-Chin Lin
    Cancer Letters
  25. Early T cell infiltration is modulated by programed cell death-1 protein and its ligand (PD-1/PD-L1) interactions in murine kidney transplants.
    Authors: Young Jun Shim, Raneem Khedraki, Jayeeta Dhar, Ran Fan, Nina Dvorina, Anna Valujskikh et al.
    Kidney International

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Reviews for Mouse Granzyme B Antibody

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Mouse Granzyme B Antibody
By Katie Myers on 11/14/2022
Application: ELISA Sample Tested: Serum Species: Mouse

This antibody worked well as both a capture and detection molecule in our mouse ELISA. it was sensitive and had little background or cross-reactivity.

ELISA Mouse Granzyme B Antibody AF1865