Mouse Hepassocin/FGL1 Antibody

Catalog #: MAB11247 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11247-100
MAB11247-SP

Detection of Hepassocin/FGL1 in Hepa 1‑6 Mouse Hepatoma Cell Line (Positive) and BaF3 Mouse Pro-B Cell Line (Negative) Cells.

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Mouse Hepassocin/FGL1 Antibody Summary

Specificity

Detects mouse FGL-1 in Direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2923B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Recombinant mouse FGL-1
Leu23-Ile314
Accession # Q71KU9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed Hepa 1‑6 Mouse Hepatoma Cell Line (Positive) and BaF3 Mouse Pro-B Cell Line (Negative) Cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Hepassocin/FGL1 in Hepa 1‑6 Mouse Hepatoma Cell Line (Positive) and BaF3 Mouse Pro-B Cell Line (Negative) Cells. Hepassocin/FGL1 was detected in immersion fixed Hepa 1‑6 Mouse Hepatoma Cell Line (Positive) and BaF3 Mouse Pro-B Cell Line (Negative) Cells using Rabbit Anti-Mouse Hepassocin/FGL1 Monoclonal Antibody (Catalog # MAB11247) at 8 µg/mL for 3 hours at room temperature. Hepa 1-6 cells were treated with 3uM Monensin for 3 hours. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background:

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein (HFREP-1) and Fibrinogen-like protein 1 (FGL-1), is a liver specific secreted protein containing one fibrinogen C-terminal domain. It exists as a homodimer and is reported to have hepatocyte mitogenic activity. Hepassocin/FGL-1 binds LAG3 independently from MHC class II (MHC-II) and acts as an Immune suppressive molecule that inhibits antigen-specific T-cell activation by acting as a major ligand of LAG3.