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Mouse IFN-beta Antibody

Catalog #: MAB8234
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8234-100
MAB8234-SP
IFN‑ beta Inhibition of Viral-induced Cytopathy and Neutralization by Mouse IFN‑ beta Antibody.
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Mouse IFN-beta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN‑ beta in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1176D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived mouse IFN‑ beta
Ile22-Asn182
Accession # P01575
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IFN‑ beta inhibition of viral-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 10 pg/mL Recombinant Mouse IFN‑ beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ beta Inhibition of Viral-induced Cytopathy and Neutralization by Mouse IFN‑ beta Antibody. View Larger

IFN‑ beta Inhibition of Viral-induced Cytopathy and Neutralization by Mouse IFN‑ beta Antibody. Recombinant Mouse IFN-beta (Catalog # 8234-MB) reduces the viral-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Inhibition of viral activity elicited by Recombinant Mouse IFN-beta (10 pg/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse IFN-beta Monoclonal Antibody (Catalog # MAB8234). The ND50 is typically 0.25-1.25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).

References
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Teige, I. et al. (2003) J. Immunol. 170:4776.
  6. Shinohara, M.L. et al. (2008) Immunity 29:68.
  7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
  11. Wang, H. et al. (2011) J. Immunol 186:675.
Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

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Citation for Mouse IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
    Authors: Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L;
    Nature communications
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo
    Applications: In vivo assay

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