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Mouse IGFBP-L1 Antibody

Catalog #: AF4130
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4130
AF4130-SP
Detection of IGFBP‑L1 in Mouse Embryo Brain.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGFBP-L1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGFBP-L1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human IGFBP-L1 is observed and less than 1% cross-reactivity with recombinant mouse IGFBP-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-L1
Leu18-Leu270
Accession # Q80W15
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IGFBP-L1 (Catalog # 4130-BL)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of mouse embryo brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of IGFBP‑L1 in Mouse Embryo Brain. IGFBP‑L1 was detected in immersion fixed paraffin-embedded sections of mouse embryo brain using Goat Anti-Mouse IGFBP‑L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4130) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-L1

Insulin-like Growth Factor Binding Protein-Like 1 (IGFBP‑L1), also known as IGFBP-rP4, is a 38 kDa secreted member of the IGFBP superfamily of proteins. IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins (1). IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region, and a C‑terminal Ig-like domain (2, 3). Mature mouse IGFBP-L1 shares 76% and 96% amino acid (aa) sequence identity with human and rat IGFBP-L1, respectively. It shares 38%-41% aa sequence identity with mouse IGFBP-7 and IGFBP-rp10 and 20%‑24% aa sequence identity with mouse IGFBP-1, -2, -3, -4, -5, and -6. IGFBP-L1 is most highly expressed in testis and brain, while lower levels are more broadly expressed (3). IGFBP-L1 is expressed in regionally and temporally distinct patterns during forebrain development (4). IGFBP-L1 inhibits cell proliferation in vitro and is downregulated in many colon and lung tumors (3). Transcriptional downregulation by promoter methylation is predictive of breast cancer aggressiveness (5).

References
  1. Firth, S.M. and R.C. Baxter (2002) Endocr. Rev. 23:824.
  2. Accession # Q80W15.
  3. Cai, Z. et al. (2005) Biochem. Biophys. Res. Commun. 331:261.
  4. Gonda, Y. et al. (2007) Gene Expr. Patterns 7:431.
  5. Smith, P. et al. (2007) Clin. Cancer Res. 13:4061.
Long Name
Insulin-like Growth Factor Binding Protein-like 1
Entrez Gene IDs
347252 (Human); 75426 (Mouse)
Alternate Names
bA113O24.1; IGFBPL1 insulin-like growth factor binding protein-like 1; IGFBPL1; IGFBP-L1; IGFBP-RP4

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Citations for Mouse IGFBP-L1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. IGFBPL1 is a master driver of microglia homeostasis and resolution of neuroinflammation in glaucoma and brain tauopathy
    Authors: Pan, L;Cho, KS;Wei, X;Xu, F;Lennikov, A;Hu, G;Tang, J;Guo, S;Chen, J;Kriukov, E;Kyle, R;Elzaridi, F;Jiang, S;Dromel, PA;Young, M;Baranov, P;Do, CW;Williams, RW;Chen, J;Lu, L;Chen, DF;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Neural precursor cells tune striatal connectivity through the release of IGFBPL1
    Authors: E Butti, S Cattaneo, M Bacigalupp, M Cambiaghi, GM Scotti, E Brambilla, F Ruffini, G Sferruzza, M Ripamonti, F Simeoni, L Cacciaguer, A Zanghì, A Quattrini, R Fesce, P Panina-Bor, F Giannese, D Cittaro, T Kuhlmann, P D'Adamo, MA Rocca, S Taverna, G Martino
    Nature Communications, 2022-12-08;13(1):7579.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  3. IGFBPL1 Regulates Axon Growth through IGF-1-mediated Signaling Cascades
    Authors: C Guo, KS Cho, Y Li, K Tchedre, C Antolik, J Ma, J Chew, TP Utheim, XA Huang, H Yu, MTA Malik, N Anzak, DF Chen
    Sci Rep, 2018-02-01;8(1):2054.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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