Mouse IL-21R Antibody

Catalog #: AF596
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF596
AF596-SP

Detection of IL‑21 R in A20 Mouse Cell Line by Flow Cytometry.

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All IL-21R Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-21R Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-21 R in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant human IL‑21 R is observed, and less than 1% cross-reactivity will recombinant mouse IL-2 R beta is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑21 R Subunit Fc Chimera (Catalog # 596-MR)

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑21 R in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL-21 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF596, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry

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IL‑10 in Mouse Splenocytes. IL‑10 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse/Rat IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells<.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-21R

The interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 R, also called NILR (novel interleukin receptor) is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 R is a 529 amino acid (aa) residue protein with a 19 aa signal peptide, a 217 aa extracellular domain, an 18 aa transmembrane domain, and a 275 aa cytoplasmic domain. Mouse and human IL-21 R share 62% aa identity. IL-21 R is expressed on lymphoid tissues, peripheral B cells, and cell lines of T, B and natural killer lineage. Although not fully elucidated, the IL-2 R gamma ( gamma _c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appears to play important roles in B and T cell proliferation after antigen stimulation and natural killer cell maturation.

References

Parrish-Novak, J. et al. (2000) Nature 408:57.
Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
Asao, H. et al. (2001) J. Immunol. 167:1.

Long Name

Interleukin 21 Receptor

Entrez Gene IDs

50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)

Alternate Names

CD360 antigen; CD360; IL-21 R; IL-21 receptor; IL21R; IL-21R; interleukin 21 receptor; interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse IL-21R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Therapeutic Effect of IL-21 Blockage by Gene Therapy in Experimental Autoimmune Encephalomyelitis
Authors: Ángel Edo, Laura Calvo-Barreiro, Herena Eixarch, Assumpció Bosch, Miguel Chillón, Carmen Espejo
Neurotherapeutics
IL-11 Induces Encephalitogenic Th17 Cells in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis
Authors: X Zhang, N Kiapour, S Kapoor, T Khan, M Thamilaras, Y Tao, S Cohen, R Miller, RA Sobel, S Markovic-P
J. Immunol., 2019-07-24;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry