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Mouse Latexin Antibody

Catalog #: AF3620 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3620
AF3620-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Latexin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Latexin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human Latexin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Latexin
Glu2-Glu222
Accession # P70202
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Latexin (Catalog # 3620-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Latexin (Catalog # 3620‑PI), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Mouse Latexin (0.1 µg/mL, Catalog # 3620-PI) inhibition of Recombinant Mouse Carboxypeptidase A1/CPA1 (0.1 µg/mL, Catalog # 2765-ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM). The Neutralization Dose (ND50) is typically 4.4 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latexin

Latexin, also known as tissue carboxypeptidase inhibitor, is the only mammalian carboxypeptidase inhibitor identified so far. Latexin was initially identified as a marker of neurons in the lateral neocortex of the developing brain (hence Latexin) (1). Further studies have shown that Latexin is highly expressed in mast cells and macrophages (2, 3). It is induced in acute pancreatitis and lung inflammatory disease. In addition, it is able to inhibit carboxypeptidases from the pancreas (CPA1 and CPA2) and mast cells (CPA3) (4). Therefore, it is thought that Latexin primarily functions in inflammation and innate immunity pathways. Compared to other carboxypeptidase inhibitors from plants and parasites, the 222 amino acid Latexin is much larger and more importantly, it lacks some conserved C-terminal residues, which interact with the target carboxypeptidase in a substrate-like manner (5). This distinct feature of Latexin suggests that it has a different carboxypeptidase inhibition mechanism.

References
  1. Arimatsu, Y. et al. (1994) Neurosci. Res. 20:131.
  2. Uratani, Y. et al. (2000) Biochem. J. 346:817.
  3. Aagaard A. et al. (2005) Structure 13:309.
  4. Normant E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:12225.
  5. Reverter D. et al. (2000) Nat. Struct. Biol. 7:322.
Entrez Gene IDs
56925 (Human); 17035 (Mouse); 59073 (Rat)
Alternate Names
ECI; ECIMUM; Endogenous carboxypeptidase inhibitor; Latexin; LXN; TCI; TCIProtein MUM; Tissue carboxypeptidase inhibitor

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