Mouse Lipocalin-13 Antibody

Catalog #: AF7974
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7974
AF7974-SP

Detection of Mouse Lipocalin-13 by Western Blot.

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All Lipocalin-13 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Lipocalin-13 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Lipocalin-13 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Lipocalin-13
Ala19-Asp176
Accession # Q8K1H9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Lipocalin-13 by Western Blot. Western blot shows lysates of mouse liver tissue, mouse skeletal muscle tissue, mouse epididymis tissue, and mouse mammary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Lipocalin-13 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF7974) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lipocalin-13 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-13

LCN-13 (Lipocalin 13; also Odorant-binding protein 2a) is a secreted, 26-28 kDa member of the LCN family, calycin superfamily of molecules. Its name is derived from Greek, and denotes the fact that lipocalins are known to be "fat-wrapping", or fat binding proteins that interact with small lipophilic molecules within a central cavity. LCN-13 is expressed by hepatocytes and smooth muscle cells. It is known to have antidiabetogenic effects, including the suppression of hepatic glucose production, the uptake of circulating glucose by adipocytes, and the induction of fatty acid oxidation by liver, possibly by stimulating CPT1 alpha production. Mouse LCN‑13 is synthesized as a 176 amino acid (aa) precursor that contains a 19 aa signal sequence and a 157 aa mature region. Although no splice forms have been reported for LCN-13 to date, a smaller, presumably proteolytically processed 23-26 kDa isoform has been noted on SDS-PAGE. Mature mouse LCN-13 shares 69% and 39% aa sequence identity with rat LCN-13 and human odorant-binding protein 2b, respectively.

Entrez Gene IDs

227627 (Mouse)

Alternate Names

Lcn;Lcn13;Obp2a;Odorant-binding protein 2a; LCN13; Lipocalin13; Lipocalin-13; Obp2a

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Lipocalin-13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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